Abstract
The bacterial strain M7D1T was isolated from samples of the rhizosphere of desert bloom plants on the Atacama region located in northern Chile as part of a study intended to isolate nitrifying bacteria in this adverse environment. It was previously identified as belonging to the Pseudomonas fluorescens group. In this study, the phylogenetic analysis of the 16s RNA, gyrA, rpoB and rpoD genes confirmed that this strain belongs to this group, especially Sub Group (SG) Koreensis, but it represents a potential new species. Additionally, the average nucleotide identity confirmed this as the highest identity value (0.92) with Pseudomonas moraviensis LMG 24280, which is lower than the 0.94 threshold established to classify two strains within the same species. The strain M7D1T shared a similar fatty acids methyl ester profile than the type strains of other Pseudomonas spp. previously described. Furthermore, it can be differentiated phenotypically from other related species of SG P. koreensis. Based on these results, the existence of a new species of Pseudomonas is demonstrated, for which the name Pseudomonas atacamensis is proposed. This strain presented a set of genes associated with plant growth-promoting rhizobacteria and it is a good candidate to be used for recovery of contaminated soils. However, more studies are required to demonstrate whether this bacterium is non-pathogenic, can survive in the presence of toxic compounds and promote growth or help to the stress management of plants.
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Acknowledgements
This work was supported by FONDEF ID17AI100007, Fondecyt iniciación 11181262 and 11170544 from National Commission for Scientific and Technological Research, (CONICYT), and a doctoral fellowship from Universidad Autónoma de Chile, Programa de Doctorado en Ciencias Biomédicas. CONICYT Proyecto Basal FB0807, CEDENNA.
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MP-M, ES and AL conceived and planned the experiments. MP-M, DC, RA, SM, CC carried out the experiments. AL took the lead in writing the manuscript. All authors provided critical feedback and helped shape the research, analysis and manuscript.
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Poblete-Morales, M., Carvajal, D., Almasia, R. et al. Pseudomonas atacamensis sp. nov., isolated from the rhizosphere of desert bloom plant in the region of Atacama, Chile. Antonie van Leeuwenhoek 113, 1201–1211 (2020). https://doi.org/10.1007/s10482-020-01427-0
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DOI: https://doi.org/10.1007/s10482-020-01427-0
Keywords
- Multilocus sequence analysis
- Genomic taxonomy
- TccC-type
- Insecticidal toxin
- Hydrogen cyanide
- PGPR
- Pseudomonadaceae