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Lactococcus protaetiae sp. nov. and Xylanimonas protaetiae sp. nov., isolated from gut of larvae of Protaetia brevitarsis seulensis

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Abstract

Two bacterial strains, designated 2DFWM-2T and FW10M-9T, were isolated from gut of larva of Protaetia brevitarsis seulensis grown at the National Institute of Agricultural Sciences, Wanju-gun, South Korea. 16S rRNA and rpoB gene sequences showed that strain 2DFWM-2T formed a separate branch with Lactococcus allomyrinae 1JSPR-7T in the genus Lactococcus, adjacent to a group of Lactococcus lactis subspecies. ANI and dDDH values between 2DFWM-2T and Lactococcus allomyrinae 1JSPR-7T were 93.30% and 53.20%, respectively. Based on the 16S rRNA gene sequence analysis, strain FW10M-9T was classified into the genus Xylanimonas revealing 96.9–98.5% sequence similarities with the Xylanimonas species. The ANI values of strain FW10M-9T with the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T and Xylanimonas cellulosilytica DSM 15894T was 81.5%, 81.2%, 81.0% and 84.1%, respectively, and the dDDH values estimated by GGDC was 24.3%, 24.3%, 29.3% and 28.1%, respectively. On the basis of the phenotypic and genotypic data, it is proposed that strain 2DFWM-2T represents a novel species of the genus Lactococcus, for which the name Lactococcus protaetiae sp. nov. is proposed, and the type strain is 2DFWM-2T (= KACC 19320T = NBRC 113069T). And, strain FW10M-9T represents a novel species of the genus Xylanimonas, for which the name Xylanimonas protaetiae sp. nov. is proposed, and the type strain is FW10M-9T (= KACC 19331T = NBRC 113053T).

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Abbreviations

MRS agar:

De Man, Rogosa and Sharpe agar

ANI:

Average nucleotide 16 identity

dDDH:

Digital DNA-DNA hybridization

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Acknowledgements

We would like to thank professor Bernhard Schink for checking the species epithet.

Funding

This study was carried out with the support (PJ013549) of National Institute of Agricultural Sciences, Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do, 55365, Republic of Korea

    Jun Heo, Soo-Jin Kim, Jeong-Seon Kim, Seung-Beom Hong & Soon-Wo Kwon

  2. Industrial Insect Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do, 55365, Republic of Korea

    Mi Ae Kim

  3. Biological Resource Center, National Institute of Technology and Evaluation (NBRC), Kazusakamatari 2-5-8, Kisarazu, Chiba, 292-0818, Japan

    Tomohiko Tamura, Satomi Saitou & Moriyuki Hamada

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  1. Jun Heo
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  4. Tomohiko Tamura
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Contributions

J. Heo, S.J. Kim, and S.W. Kwon designed reseach: M.A. Kim provided the insect sample; T. Tamura, S. Saitou, and M. Hamada carried out the chemotaxonomic analysis: J.S. Kim and S.B. Hong participated experiments; Soon-Wo Kwon wrote the paper; all authors provided critical feedback and helped shape the research, analysis and manuscript.

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Correspondence to Soon-Wo Kwon.

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This article does not contain any studies with human participants and/or animals performed by any of the authors. The formal consent is not required in this study.

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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence and genome sequence of strain 2DFWM-2T and FW10M-9T are MN121516 and CP041356, and MK490978 and CP035493, respectively.

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Heo, J., Kim, SJ., Kim, M.A. et al. Lactococcus protaetiae sp. nov. and Xylanimonas protaetiae sp. nov., isolated from gut of larvae of Protaetia brevitarsis seulensis. Antonie van Leeuwenhoek 113, 1009–1021 (2020). https://doi.org/10.1007/s10482-020-01413-6

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