Abstract
An alkaliphilic and moderately halophilic strain, designated YSP-3T, characterised by optimal growth at pH 9.0 and at 8.0% (w/v) NaCl, was isolated from Yangshapao Lake, Jilin Province, China. Cells of this strain is Gram-positive, straight rods and form a central or sub-terminal ellipsoidal endospore. Phylogenetic analysis based on 16S rRNA gene sequences indicated that it was grouped in the genus Bacillus with Bacillus aurantiacus K1-5T and Bacillus populi FJAT-45347T as the close relative (97.5 and 97.2% 16S rRNA gene sequence similarity, respectively). Genomic relatedness between strain YSP-3T and its close relative was evaluated using average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity with the values of 70.3–85.1%, 19.7–20.1% and 71.5–71.6%, respectively. Comparative genomics analysis showed that strain YSP-3T has distinct amino acid bias and significantly differences from foreign invasion events during evolution relative to the reference strains. Cell-wall peptidoglycan contains meso-diaminopimelic acid. The predominant polar lipids are phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol. The predominant quinone is menaquinone-7. The major fatty acids of strain YSP-3T are anteiso-C15:0, iso-C15:0, iso-C16:0, anteiso-C17:0 and Iso-C14:0. DNA G + C content of strain YSP-3T is 48.3 mol%. Based on genomics analysis, physiological, biochemical and chemotaxonomic data, strain YSP-3T represent a novel species, for which the name Bacillus lacisalsi sp. nov. is proposed. The type strain is YSP-3T ( = ACCC 60365T = KCTC 33934T).
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Acknowledgements
This work was supported by National Natural Science Foundation of China (Grant No. 31300101) and Fundamental Research Funds for Central Non-profit Scientific Institution (Grant No. 1610042018005).
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DL and WS wrote the main manuscript text. WH designed the experiments. DL and WS carried out the experiments. WH, ZB and ZX analysed the data. All authors approved and read the final manuscript.
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Dong, L., Wang, S., Cao, H. et al. Bacillus lacisalsi sp. nov., a moderately haloalkaliphilic bacterium isolated from a saline–alkaline lake. Antonie van Leeuwenhoek 113, 127–136 (2020). https://doi.org/10.1007/s10482-019-01322-3
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DOI: https://doi.org/10.1007/s10482-019-01322-3