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Microlunatus speluncae sp. nov., a novel actinobacterium isolated from a Karstic subterranean environment sample

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Abstract

A novel actinobacterial strain, designated SYSU K12189T, was isolated from a soil sample collected from a Karst cave in Xingyi county, Guizhou province, south-western China. The taxonomic position of the strain was investigated using a polyphasic approach. Cells of the strain were observed to be aerobic and Gram-stain positive. On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU K12189T is closely related to the type strains of the genus Microlunatus, Microlunatus parietis 12-Be-011T (98.5% sequence similarity), Microlunatus nigridraconis CPCC 203993T (98.4%) and Microlunatus cavernae YIM C01117T (96.6%), and is therefore considered to represent a member of the genus Microlunatus. DNA–DNA hybridization values between strain SYSU K12189T and related type strains of the genus Microlunatus were < 70%. In addition, LL-diaminopimelic acid was found to be the diagnostic diamino acid in the cell wall peptidoglycan. The major isoprenoid quinone was identified as MK-9(H4), while the major fatty acids (> 10%) were found to be anteiso-C15:0, iso-C15:0, iso-C16:0 and iso-C14:0. The polar lipids were found to contain diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, three glycolipids and two unidentified lipids. The genomic DNA G+C content of strain SYSU K12189T was determined to be 69.4 mol%. On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K12189T is concluded to represent a novel species of the genus Microlunatus, for which the name Microlunatus speluncae sp. nov. is proposed. The type strain is SYSU K12189T (= KCTC 39847T = DSM 103947T).

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Funding

This research was supported by National Key R&D Program of China (2017YFD0200503), Natural Science Foundation of China (No. 31600015) and National Fundamental Fund Project Subsidy Funds of Personnel Training of China (No. J1310025).

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Author notes

  1. Yuan-Guo Xie and Bao-Zhu Fang have contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

    Yuan-Guo Xie, Bao-Zhu Fang, Ming-Xian Han, Lan Liu, Jian-Yu Jiao, Xiao-Tong Zhang, Min Xiao & Wen-Jun Li

  2. State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, 430074, People’s Republic of China

    Ming-Xian Han

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  1. Yuan-Guo Xie
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Contributions

WJL, YGX and BZF designed research and project outline. MXH, LK LL and YGX performed isolation, deposition and identification. JYJ and XTZ performed genome analysis. BZF and WJL drafted the manuscript. All authors read and approved the final manuscript.

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Correspondence to Bao-Zhu Fang or Wen-Jun Li.

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Xie, YG., Fang, BZ., Han, MX. et al. Microlunatus speluncae sp. nov., a novel actinobacterium isolated from a Karstic subterranean environment sample. Antonie van Leeuwenhoek 113, 117–125 (2020). https://doi.org/10.1007/s10482-019-01321-4

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