Yeast communities of primary and secondary peat swamp forests in southern Thailand
Khanthuli peat swamp forest (PSF) is one of a few fertile peat swamp forests that remain in Thailand. It is composed of primary PSF and some areas which have been degraded to secondary PSF due to drought, wildfires and land conversion, which have resulted in a decrease in peat layers and change in the species of the plant community. In this study, diversity of yeasts in peat from both primary and secondary PSF areas of the Khanthuli PSF was determined based on culture-dependent approaches, using dilution plate and enrichment techniques. A total of 66 yeast isolates were identified by the analysis of sequence similarity of the D1/D2 region of the large subunit rRNA gene or the combined analysis of sequence of the D1/D2 region and internal transcribed spacer region and confirmed by phylogenetic analysis of the D1/D2 region to belong to 22 known yeast species and six potential new species in the genera Candida (Kurtzmaniella, Lodderomyces, Ogataea, Pichia and Yamadazyma clades), Clavispora, Cyberlindnera, Galactomyces, Hanseniaspora, Metschnikowia, Saturnispora, Schwanniomyces, Cryptotrichosporon, Pichia, Curvibasidium, Papiliotrema, Rhodotorula, and Saitozyma. The most prevalent yeasts in the primary PSF were Cyberlindnera subsufficiens and Galactomyces candidus, while Saitozyma podzolica was the most frequently found in peat from the secondary PSF. Common yeast species in both, primary and secondary PSF, were Cy. subsufficiens, G. candidus and Rhodotorula mucilaginosa.
KeywordsPeat swamp forests Thailand Yeast diversity
CB: Data analysis and wrote the manuscript. PK: Performed experiments and data analysis. SL: Designed study, data discussion and editing the manuscript.
This work was supported by the Center of Excellence on Biodiversity, Postgraduate Education and Research Development Office (PERDO) through the Project ‘Diversity of yeast in peat swamp forest and their potential in agricultural application’ Grant No. BDC-PG1-160001.
Compliance with ethical standards
Conflicts of interest
The authors declare that there are no conflicts of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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