Lactobacillus jinshani sp. nov., isolated from solid-state vinegar culture of Zhenjiang aromatic vinegar
- 83 Downloads
A novel Gram-stain-positive, non-motile, non-spore-forming, rod-shaped, facultatively anaerobic, designated strain HSLZ-75T, was isolated from the solid-state vinegar culture of Zhenjiang aromatic vinegar. Strain HSLZ-75T grew at 20–40 °C (optimum 35 °C), pH 3.0–5.0 (optimum pH 4.0) and 0–5% (w/v) NaCl (optimum 0%). Heterolactic fermentation characterised the metabolism of strain HSLZ-75T. d- and l-lactic acid were produced from glucose in a ratio of 91:9. The major cellular fatty acids ( > 10%) consisted of C16:0, C18:1ω9c, summed feature 8 (C18:1ω7c and/or C18:1ω6c) and C19:0 cyclo ω8c. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified phospholipid and six unknown lipids. The cell wall was found to contain meso-diaminopimelic acid-type peptidoglycan. The 16S rRNA gene sequence of strain HSLZ-75T showed the highest similarity of 88.0% with Lactobacillus fructivorans DSM 20203T. Phylogenetic analysis indicated that strain HSLZ-75T belonged to family Lactobacillaceae and formed a distinct lineage with the type strain of Lactobacillus caviae. The complete genome of strain HSLZ-75T contained a circular chromosome of 1,616,430 bp with 1570 genes and 39.7 mol% G + C content. The average nucleotide identity values between strain HSLZ-75T and the reference type strains Lactobacillus fructivorans DSM 20203T and Lactobacillus rossiae DSM 15814T were 66.4% and 65.7%, respectively. On the basis of phenotypic, chemotaxonomic, phylogenetic and genotypic characteristics, strain HSLZ-75T should be classified as a novel species of the genus Lactobacillus in the family Lactobacillaceae of the order Lactobacillales, for which the name Lactobacillus jinshani sp. nov. is proposed. The type strain is HSLZ-75T ( = CICC 6269T = JCM 33270T).
KeywordsLactobacillus jinshani Zhenjiang aromatic vinegar Lactic acid bacteria
We would like to express our gratitude to Prof. Henglin Cui for helpful discussions, are grateful to Prof. Aharon Oren (the Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem) for the suggestions for the nomination in this study. This study was financially supported by the National key research and development program of China (No. 2018YFD0400401) and the Key research and development program of Zhenjiang (No. Y2017011).
YY, ZML and ZHX designed the experiments. XL, JZ and LJC conducted the experiments. XL, JZ, LJC and ZML analysed the results. YY, XL, JZ, LJC, ZML and ZHX prepared the manuscript. All authors read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that there is no conflict of interest.
The article does not contain any studies related to human participants or animals.
- Dellaglio F, Trovatelli LD, Sarra PG (1981) DNA–DNA homology among representative strains of the genus Pediococcus. Zbl Bakt Hyg I Abt Orig C 2:140–150Google Scholar
- Killer J, Pechar R, Svec P, Salmonová H, Svejstil R, Geigerová M, Rada V, Vlková E, Mekadim C (2017) Lactobacillus caviae sp. nov., an obligately heterofermentative bacterium isolated from the oral cavity of a guinea pig (Cavia aperea f. porcellus). Int J Syst Evol Microbiol 67:2903–2909CrossRefPubMedGoogle Scholar
- Kozaki M, Uchimura T, Okada S (1992) Experimental manual of lactic acid bacteria. Asakurasyoten, Tokyo, pp 29–72Google Scholar
- Miyashita M, Yukphan P, Chaipitakchonlatarn W, Malimas T, Sugimoto M, Yoshino M, Kamakura Y, Potacharoen W, Tanasupawat S, Tanaka N, Nakagawa Y, Suzuki K (2015) Lactobacillus plajomi sp. nov. and Lactobacillus modestisalitolerans sp. nov., isolated from traditional fermented foods. Int J Syst Evol Microbiol 65:2485–2490CrossRefPubMedGoogle Scholar
- Rzhetsky A, Nei M (1992) A simple method for evaluating and testing minimum-evolution trees. Mol Biol Evol 9:945–967Google Scholar
- Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425Google Scholar
- Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. Technical Note 101. Microbial ID, Newark, DEGoogle Scholar
- Tanasupawat S, Pakdeeto A, Thawai C, Yukphan P, Okada S (2007) Identification of lactic acid bacteria from fermented tea leaves (miang) in Thailand and proposals of Lactobacillus thailandensis sp. nov. Lactobacillus camelliae sp. nov., and Pediococcus siamensis sp. nov. J Gen Appl Microbiol 53:7–15CrossRefPubMedGoogle Scholar
- Weiss N, Schillinger U, Laternser M, Kandler O (1981) Lactobacillus sharpeae sp. nov. and Lactobacillus agilis sp. nov., two new species of homofermentative, meso-diaminopimelic acid containing lactobacilli isolated from sewage. Zbl Bakt Hyg I Abt Orig C 2:242–253Google Scholar