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Thermus caldilimi sp. nov., a thermophilic bacterium isolated from a geothermal area

  • Meng-Meng Li
  • Wen-Dong Xian
  • Xiao-Tong Zhang
  • Yi-Rui Yin
  • En-Min Zhou
  • Yi-Ping Ding
  • Lan Liu
  • Bao-Zhu Fang
  • Wen-Jun LiEmail author
Original Paper
  • 48 Downloads

Abstract

A Gram-stain negative, aerobic bacterium, designated strain YIM 78456T, was isolated from a hot spring sediment, Ngamring county, Tibet, south-west China. The taxonomic position of the isolate was investigated by a polyphasic approach. The novel isolate was found to be aerobic and rod-shaped. Colonies were observed to be pale yellow and circular. The strain was found to grow at pH 7.0–8.0 (optimum, pH 7.0), 45–65 °C (optimum, 55 °C) and in the presence of up to 1.5% NaCl. Comparison of the 16S rRNA gene sequence of strain YIM 78456T and other members of the genus Thermus showed sequence similarities ranging from 90.3 to 97.3%, with strain YIM 78456T showing close sequence similarity to Thermus caliditerrae YIM 77925T (97.3%). The phylogenetic trees based on 16S rRNA gene sequences showed that strain YIM 78456T forms a distinct clade with T. caliditerrae YIM 77925T. The predominant menaquinone was identified as MK-8 and the DNA G+C content was determined to be 65.1 mol%. The major cellular fatty acids (> 10%) were identified as iso-C15:0, anteiso-C15:0 and iso-C17:0. The polar lipids were found to consist of an aminophospholipid, a phospholipid and glycolipids. On the basis of the morphological and chemotaxonomic characteristics, as well as genotypic data, it is proposed that strain YIM 78456T represents a novel species of the genus Thermus, for which the name Thermus caldilimi sp. nov. is proposed. The type strain is YIM 78456T (= KCTC 52948T = NBRC 113036T).

Keywords

Thermus caldilimi sp. nov. Hot spring Tibet Polyphasic approach 

Notes

Acknowledgements

This research was supported by China Ministry of Science and Technology (No. 2015FY110100), Natural Science Foundation of China (No. 31600103) and Natural Science Foundation of Guangdong Province, China (No. 2016A030312003).

Author contributions

WJL conceived the study. MML, BZF, XTZ, YRY, EMZ, YPD, LL performed research. MML, WDX and XTZ analyzed data. MML, WDX and WJL wrote the paper. All authors approved the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no direct or indirect conflict of interest.

Ethical statement

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

10482_2019_1309_MOESM1_ESM.docx (807 kb)
Supplementary material 1 (DOCX 806 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life SciencesSun Yat-Sen UniversityGuangzhouPeople’s Republic of China
  2. 2.School of Resource Environment and Earth ScienceYunnan UniversityKunmingPeople’s Republic of China

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