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Ilyomonas limi gen. nov., sp. nov., a new member of the family Chitinophagaceae isolated from mud

  • Geeta Chhetri
  • Jiyoun Kim
  • Hyungdong Kim
  • Inhyup Kim
  • Myung Kyum Kim
  • Taegun SeoEmail author
Original Paper

Abstract

A Gram-strain negative, aerobic, catalase and oxidase positive, non-motile, short rod-shaped bacterium, designated 17mud1-8T, was isolated from mud collected from Nowon-gu, Seoul, South Korea. The strain was found to be able to grow at 10–40 °C (optimum 28–30 °C), pH 5.0–8.0 (optimum 7.0), and in the absence of NaCl. The nearly full-length 16S rRNA gene of strain 17mud1-8T exhibits sequence similarity of 94.1% with that of Panacibacter ginsenosidivorans Gsoil 1550T, followed by 93.6% sequence similarity with Parafilimonas terrae DSM 28286T. Phylogenetic analysis indicated that strain 17mud1-8T belongs to the family Chitinophagaceae, sharing approximately 94.1–91.9% sequence similarity with members of closely related genera. The respiratory quinone was identified as MK-7. The predominant fatty acids were found to consist of iso-C15:0, iso-C17:1ω5c and iso-C15:1 G. The polar lipids were identified as phosphatidylethanolamine, an unidentified aminophospholipid, an unidentified glycolipid, ten unidentified aminolipids and seven unidentified lipids. The draft genome of 17mud1-8T has G+C content of 40.9 mol% and a 5.8 Mb chromosome. On the basis of the phenotypic and genotypic properties, and phylogenetic inference, strain 17mud1-8T was found to represent a novel genus in the family Chitinophagaceae, for which the name Ilyomonas limi gen. nov., sp. nov. is proposed, with the type strain 17mud1-8T(=KCTC 52874T = NBRC 112826T).

Keywords

Chitinophagaceae Phylogenetic analysis Cell morphology Polyphasic taxonomy 

Notes

Acknowledgements

This work was supported by a research grant from Seoul Women’s University (2018) and the Project on Survey of Indigenous Species of Korea of the National Institute of Biological Resources (NIBR) under the Ministry of Environment (MOE) (NIBR201701206). We thank Prof Dr. Bernhard Schink (University of Konstanz, Konstanz, Germany) for advice concerning the species name.

Authors’ contribution

GC isolated the bacterium, designed the study, performed the phenotypic and biochemical characterization, wrote the original draft; JK, HK and IK helped the analysis of taxonomic data; MKK and TS designed and supervised the study, edited the original draft.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.

Ethical standards

This study does not describe any experimental work related to human.

Supplementary material

10482_2019_1300_MOESM1_ESM.docx (5.6 mb)
Supplementary material 1 (DOCX 5781 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Geeta Chhetri
    • 1
  • Jiyoun Kim
    • 1
  • Hyungdong Kim
    • 1
  • Inhyup Kim
    • 1
  • Myung Kyum Kim
    • 2
  • Taegun Seo
    • 1
    Email author
  1. 1.Department of Life ScienceDongguk University-SeoulGoyangSouth Korea
  2. 2.Department of Bio and Environmental Technology, College of Natural ScienceSeoul Women’s UniversitySeoulSouth Korea

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