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Antonie van Leeuwenhoek

, Volume 112, Issue 11, pp 1699–1704 | Cite as

Flavobacterium sangjuense sp. nov. isolated from sediment

  • Hyangmi KimEmail author
  • Sun-Yeon Heo
  • Jee-Hwan Kim
  • Jong-Chan Chae
  • Sang Mi YuEmail author
Original Paper
  • 72 Downloads

Abstract

A yellow-pigmented bacterial strain, GS03T, was isolated from sediment in a branch of the Nackdong River in Sangju, Korea. Cells were observed to be Gram-negative, aerobic and rod-shaped with gliding motility, and to be positive for catalase and oxidase. Growth was found to occur at 4–30 °C (optimum 25 °C), at pH 7.0–8.5 (optimum pH 7.5) and at NaCl 0% (optimum NaCl 0%, w/v). The major cellular fatty acids (> 10% of the total) were identified as iso C15:0, iso C15:1 G, C15:1ω6c, iso C15: 0 3-OH and iso C17: 0 3-OH. The major respiratory quinone was found to be menaquinone MK-6. The genome sequence of GS03T is 3.1 Mb with G+C content of 36.1 mol%. The major polar lipids of the isolate were identified as phosphatidylethanolamine, three unidentified aminolipids, two unidentified phospholipids, an unidentified lipid and an unidentified aminophospholipid. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain GS03T clusters with Flavobacterium paronense KNUS1TT, with similarity of 96.8%. The phenotypic, phylogenetic, and chemotaxonomic characteristics indicate that strain GS03T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium sangjuense sp. nov. is proposed. The type strain is GS03T (= FBCC 502459T = KCTC 62568T = JCM 32764T).

Keywords

Fresh water Flavobacterium Flavobacteriaceae 

Notes

Acknowledgements

This work was supported by a Grant from the Nakdonggang National Institute of Biological Resources (NNIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NNIBR201902106).

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.

Supplementary material

10482_2019_1297_MOESM1_ESM.docx (362 kb)
Supplementary material 1 (DOCX 362 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Bacteria Research TeamNNIBRSangjuRepublic of Korea
  2. 2.Microbial Biotechnology Research CenterKRIBBJeong-upRepublic of Korea
  3. 3.Bioresources Industrialization Support DepartmentSangjuRepublic of Korea
  4. 4.Division of BiotechnologyChonbuk National UniversityIksanRepublic of Korea
  5. 5.Environmental Microbiology Research TeamNNIBRSangjuRepublic of Korea

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