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Antonie van Leeuwenhoek

, Volume 112, Issue 11, pp 1675–1689 | Cite as

Comparative transcriptomic analysis identified differentially expressed genes and pathways involved in the interaction between Tremella fuciformis and Annulohypoxylon stygium

  • Dongmei Liu
  • Dwi Pujiana
  • Yuanyuan Wang
  • Zhaosong Zhang
  • Liesheng Zheng
  • Liguo Chen
  • Aimin MaEmail author
Original Paper
  • 105 Downloads

Abstract

Tremella fuciformis is an edible and medicinal white jelly mushroom. It has a life cycle that interacts with its companion fungus Annulohypoxylon stygium, both in natural conditions and artificial cultivation. RNA sequencing (RNA-Seq) was used to study the interaction between T. fuciformis and A. stygium by constructing 5 libraries, including the individual T. fuciformis mycelium (1), the T. fuciformis mycelium after interaction with A. stygium (2), the dual mycelia after interaction (3), the A. stygium mycelium after interaction with T. fuciformis (4), and the individual A. stygium mycelium (5). 33.4 G data and 46,871 Unigenes were generated from de novo splicing. For identification of differentially expressed genes (DEGs) related to interaction, we analyzed the expression data of DEGs1-vs-2 ∩ DEGs1-vs-3, and DEGs5-vs-4 ∩ DEGs5-vs-3. DEGs1-vs-2 ∩ DEGs1-vs-3, and DEGs5-vs-4 ∩ DEGs5-vs-3 data showed 614 DEGs and 1537 DEGs, respectively. The 614 DEGs for T. fuciformis and 1537 DEGs for A. stygium were analyzed by GO annotation and were assigned to biology process, cell composition, and molecular functions. The DEGs were used to match the KEGG database. In T. fuciformis, the pathways are primarily enriched various amino acids metabolism, pentose and glucuronate interconversions. In A. stygium, the pathways are primarily enriched in the biosynthesis of secondary metabolites, biosynthesis of antibiotics, starch and sucrose metabolism. The expression patterns of DEGs determined by qRT-PCR were consistent with those obtained by RNA-Seq, thus validating the reliability of our RNA-Seq data. Future studies of the functions of these interesting genes will be helpful to understand the mechanisms by which T. fuciformis interacts with A. stygium. This will also provide a reference for other research on interacting microorganisms.

Keywords

Tremella fuciformis Annulohypoxylon stygium Interaction RNA sequencing Differentially expressed genes Pathway 

Notes

Acknowledgements

This research was supported by a grant from the National Natural Science Foundation of China (NSFC) (No. 31572182) to Aimin Ma.

Author’s contribution

DL and AM designed the experiments. DL and DP performed the experiments. YW and ZZ analyzed the data. DL wrote the manuscript. LZ, LC, and AM critically reviewed the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Dongmei Liu
    • 1
  • Dwi Pujiana
    • 1
  • Yuanyuan Wang
    • 1
  • Zhaosong Zhang
    • 1
  • Liesheng Zheng
    • 2
  • Liguo Chen
    • 2
  • Aimin Ma
    • 1
    • 3
    Email author
  1. 1.College of Food Science and TechnologyHuazhong Agricultural UniversityWuhanChina
  2. 2.College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanChina
  3. 3.Key Laboratory of Agro-Microbial Resources and UtilizationMinistry of AgricultureWuhanChina

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