Antonie van Leeuwenhoek

, Volume 112, Issue 11, pp 1633–1644 | Cite as

Co-inoculation of different antagonists can enhance the biocontrol activity against Rhizoctonia solani in tomato

  • Manoj Kumar Solanki
  • Mahesh S. YandigeriEmail author
  • Sudheer Kumar
  • Rajesh Kumar Singh
  • Alok K. Srivastava
Original Paper


Biological control by using microbial inoculants is adopted as the best alternative to chemical pesticides to manage plant diseases. In the present study, a microbial consortia based management strategy involving the microbes Bacillus velezensis MB101 (BV), Streptomyces atrovirens N23 (SA) and Trichoderma lixii NAIMCC-F-01760 (TL), was evaluated for the management of Rhizoctonia solani (RS), the causal agent of tomato root rot. The efficacy of these microbial inoculants was evaluated in glasshouse and field experiments. Plant defense-related enzymes were assayed in the glasshouse, and biocontrol effect was evaluated in the field with RS infected soil. In the glasshouse experiment, co-inoculated SA + TL treated plants showed maximum disease resistance in comparison to control. Also, the plant defense-related enzymes such as chitinase, β-1,3-glucanase, peroxidases, polyphenol oxidase, and phenylalanine ammonia lyase were increased in this treatment. Furthermore, three application methods were assessed in the field, and SA + TL showed maximum disease reduction (76%) by the dual application. Based on glasshouse and field study results, it was concluded that co-inoculation of SA + TL activated plant defense against RS as compared to the individual microbes, and co-inoculation could be a new effective strategy to manage the root rot pathogen in an eco-compatible manner.


Bio-control Root rot Bacillus velezensis Trichoderma lixii Streptomyces atrovirens Plant defense Rhizoctonia solani Tomato 



This work was funded by the Indian Council of Agriculture Research (ICAR) by a network project ‘Application of Microorganisms in Agriculture and Allied Sectors’ (AMAAS). Microbial culture collection unit (NAIMCC) of ICAR-NBAIM is highly appreciable for providing cultures for this study.

Author contributions

Conceptualization, MKS, SK, and MSY; Experiment setup, data collection and analysis MKS, and RKS; Funding acquisition, SK and MSY; Project administration and supervision, SK and AKS; Writing—original draft, MKS, RKS and MSY, Writing—review and editing, SK and AKS

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

10482_2019_1290_MOESM1_ESM.doc (80 kb)
Supplementary material 1 (DOC 80 kb)


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.ICAR-National Bureau of Agriculturally Important MicroorganismsKusmaur, MauIndia
  2. 2.ICAR-National Bureau of Agricultural Insect ResourcesBangaloreIndia
  3. 3.ICAR-Indian Institute of Wheat and Barley ResearchKarnalIndia
  4. 4.Department of Postharvest and Food Sciences, The Volcani CenterAgricultural Research OrganizationRishon LeZionIsrael

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