Cryobacterium tepidiphilum sp. nov., isolated from rhizosphere soil of lettuce (var. ramosa Hort.)
A Gram-stain positive, aerobic, rod-shaped bacterium, designated strain NEAU-85T, was isolated from rhizosphere soil of lettuce and characterised using a polyphasic approach. Strain NEAU-85T was found to be catalase positive, motile and able to grow at between 10 and 30 °C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain NEAU-85T belongs to the genus Cryobacterium and is closely related to the type strain Cryobacterium psychrotolerans JCM 13925T (98.5%) and also to Leifsonia kafniensis NCCB 100216T (97.6%). Multilocus sequence analysis using the concatenated gene sequences of the atpD, dnaK, recA, rpoB, sevY and ychF genes showed that strain NEAU-85T belongs to the genus Cryobacterium. The digital DNA–DNA hybridization values revealed that strain NEAU-85T is distinct from its close phylogenetic neighbour C. psychrotolerans. The menaquinones were identified as MK-10 and MK-11. The phospholipid profile was found to consist of diphosphatidylglycerol, phosphatidylglycerol, phospholipid, an unidentified glycolipid and an unidentified lipid. The major fatty acids were identified as anteiso-C15:0, anteiso-C15:1 and iso-C16:0. The genomic DNA G + C content of strain NEAU-85T was determined to be 68.9 mol%. The DNA–DNA hybridization value between them was less than 70%. On the basis of phenotypic, genotypic and phylogenetic data, strain NEAU-85T can be concluded to represent a novel species of the genus Cryobacterium, for which the name Cryobacterium tepidiphilum sp. nov. is proposed. The type strain is NEAU-85T (= CCTCC AA 2018035T = JCM 32545T).
KeywordsCryobacterium tepidiphilum sp. nov. Polyphasic analysis 16S rRNA gene Rhizosphere soil of lettuce
Multilocus sequence analysis
ATP synthase, beta subunit
Chaperone protein DnaK
Homologous recombination factor
RNA polymerase, beta subunit
Protein translocase subunit
GTP-binding and nucleic acid-binding protein
International Streptomyces Project
Inter-society color council-national bureau of standards
Gas chromatography–mass spectrometer
Molecular evolutionary genetics analysis
China center for type culture collection
Japan collection of microorganisms
This work was supported in part by grants from the National Natural Science Foundation of China (No. 31772240). We are grateful to Prof. Aharon Oren for helpful advice on the specific epithet.
YW performed the laboratory experiments, analysed the data, and drafted the manuscript with PC. PS contributed to the biochemical characterisation. XS contributed to the polyphasic taxonomy. CL contributed to the morphological analyses. JZ contributed to the fatty acids determination. JZ participated in the discussions of each section of experiments. XW and WX designed the experiments and revised the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants and/or animals performed by any of the authors. The formal consent is not required in this study.
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