A polyphasic taxonomic approach was used to characterize a presumptively novel diazotrophic bacterium, designated strain CC-YTH430T, isolated from a compost sample in Taiwan. Cells of strain CC-YTH430T were found to be Gram-stain negative, facultative anaerobic rods that formed yellow-colored colonies on nutrient agar. Cell growth occurred at 15–40 °C, pH 5.0–9.0 and in the presence of 0–2% NaCl. Strain CC-YTH430T resembled Mesorhizobium species while sharing high pair-wise 16S rRNA gene sequence similarities with Mesorhizobium silamurunense, Mesorhizobium thiogangeticum, Mesorhizobium plurifarium, Mesorhizobium tamadayense, Mesorhizobium amorphae (96.9% each), Mesorhizobium sediminum (96.8%), and Mesorhizobium soli (96.5%) and < 96.5% similarity to other species. Strain CC-YTH430T showed 78.8–79.7% average nucleotide identity compared to the type strains of M. amorphae, M. plurifarium, M. soli, M. tamadayense and M. wenxiniae. The N2-fixing activity of strain CC-YTH430T was 0.2 nmol ethylene h−1 at 30 °C. The respiratory system was ubiquinone 10 (Q-10) and the DNA G+C content was 62.0 ± 0.2 mol%. The major fatty acids (> 5%) were C16:0, C17:0 cyclo, C19:0 cyclo ω8c, C14:0 3OH/C16:1 iso I and C18:1ω7c/C18:1ω6c. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine and an unidentified aminolipid in major amounts. In addition, phosphatidylethanolamine, an unidentified lipid and several unidentified polar lipids were also found in moderate-to-trace amounts. Based on the phylogenetic, phenotypic and chemotaxonomic features, strain CC-YTH430T is proposed to represent a novel Mesorhizobium species, for which the name Mesorhizobium composti sp. nov. (type strain CC-YTH430T = BCRC 81024T = JCM 31762T) is proposed.
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Average nucleotide identity
Bioresource Collection and Research Center
Deutsche Sammlung von Mikroorganismen und Zellkulturen
Fatty acid methyl esters
High-performance liquid chromatography
Japan Collection of Microorganisms
Korean Collection for Type Cultures
Tryptic soy agar
Yeast extract mannitol agar
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This work was financially supported by the Ministry of Science and Technology (Taiwan) under Grant No. MOST 107-2634-F-005-002 and by the “Innovation and Development Center of Sustainable Agriculture” from The Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE) in Taiwan.
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The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, atpD, recA gene sequences and the draft genome of Mesorhizobium composti strain CC-YTH430T are KX988315, MG708501, MG708502 and SSNY00000000, respectively.
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Lin, SY., Hameed, A., Hsieh, YT. et al. Mesorhizobium composti sp. nov., isolated from compost. Antonie van Leeuwenhoek 112, 1387–1398 (2019). https://doi.org/10.1007/s10482-019-01270-y
- Mesorhizobium composti
- Polyphasic taxonomy