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Aestuariisphingobium litorale gen. nov., sp. nov., a novel proteobacterium isolated from a water sample of Pearl River estuary

Abstract

Strain SYSU M10002T was isolated from a water sample collected from the coastal region of Pearl River estuary, Guangdong Province, southern China. The taxonomic position of the isolate was investigated by polyphasic taxonomic approaches. The isolate was found to be Gram-negative, non-motile, short rods and aerobic. The strain was able to grow at 14–37 °C, pH 6.0–10.0 and in the presence of up to 0.5% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SYSU M10002T is a member of the family Sphingomonadaceae, with high sequence similarity to Sphingorhabdus buctiana T5T (95.1%). Overall genomic related indices between the genome of strain SYSU M10002T and those of related strains were low to moderate (AAI values < 64.3%; POCP values < 58%), indicating that strain SYSU M10002T represents a novel lineage within the family Sphinogomonadaceae. Strain SYSU M10002T contained homospermidine as its polyamine. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid, two unidentified phospholipids and an unidentified aminolipid. Ubiquinone Q-9 (44.9%) and Q-10 (43.2%) were the dominant respiratory quinones, along with a minor amount of Q-8 (11.9%). The predominant cellular fatty acids (> 10%) identified were summed feature 3 (C16:1ω7c and/or C16:1ω6c), summed feature 8 (C18:1ω7c) and C14:0 2-OH. The genomic DNA G+C content was 64.0%. Based on the analyses of the phenotypic, genotypic and phylogenetic characteristics, strain SYSU M10002T is determined to represent a novel species of a novel genus, for which the name Aestuariisphingobium litorale gen. nov., sp. nov. is proposed. The type strain of the species is SYSU M10002T (= KCTC 52944T = NBRC 112961T).

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Acknowledgements

This research was supported by the Natural Science Foundation of China (No. 31528001). X. Mou was supported by the Ohio Board of Regents (sub-award no. 60049296). W.-J. Li was also supported by Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme (2014).

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N.S. and W.J.L. conceived the study. X.L., J.L.L, X.T.Z., N.S., L.D. and M.D.A. performed research. N.S., X.M. and M.X. analyzed data. X.L., N.S. and W.J.L. wrote the paper. All authors approved the manuscript.

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Correspondence to Nimaichand Salam or Wen-Jun Li.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Li, X., Li, JL., Zhang, XT. et al. Aestuariisphingobium litorale gen. nov., sp. nov., a novel proteobacterium isolated from a water sample of Pearl River estuary. Antonie van Leeuwenhoek 112, 1357–1367 (2019). https://doi.org/10.1007/s10482-019-01268-6

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  • DOI: https://doi.org/10.1007/s10482-019-01268-6

Keywords

  • Aestuariisphingobium litorale gen. nov., sp. nov.
  • Polyphasic taxonomy
  • Pearl River estuary