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Pseudoxanthomonas composti sp. nov., isolated from compost

Abstract

A Gram-staining negative bacterium, designated as GSS15T, was isolated from compost in Guangzhou, China. Cells of strain GSS15T were rod-shaped and non-motile. The isolate was able to grow at 15–42 °C (optimum 30 °C) and pH 6.0–11.0 (optimum pH 8.0), and tolerate up to 6.0% NaCl (w/v). When the 16S rRNA gene sequence of the isolate was compared with those of other bacteria, the highest similarity was observed with Pseudoxanthomonas helianthi roo10T (96.9%). Furthermore, strain GSS15T showed low ANI (75.7–79.5%) and DDH (24.2–18.3%) values to the closely related species. Q-8 was the predominant respiratory quinone. The major cellular fatty acids ( > 5%) were iso-C15:0 (18.7%), C16:1ω7c (18.6%), anteiso-C15:0 (13.2%), C16:0 (9.8%), and iso-C16:0 (8.8%). The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. Based on its phenotypic, chemotaxonomic and genotypic data, strain GSS15T (= KCTC 52974T = MCCC 1K03334T) is designated as the type strain of a novel species of the genus Pseudoxanthomonas, for which the name Pseudoxanthomonas composti sp. nov. is proposed.

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Acknowledgements

Financial support was provided by the GDAS' Project of Science and Technology Development (No. 2019GDASYL-0501005), the Science and Technology Planning Project of Guangdong Province (Nos. 2017A030303057, 2017A070702015), and the Scientific Research Project of Education Department of Hunan Province (No. 13C019).

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ZY and LZ designed research and project outline and drafted the manuscript. JL, GY performed isolation, deposition and identification. JT and ZL performed genome analysis. All authors read and approved the final manuscript.

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Correspondence to Zhen Yu or Li Zhuang.

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Lin, J., Yang, G., Tang, J. et al. Pseudoxanthomonas composti sp. nov., isolated from compost. Antonie van Leeuwenhoek 112, 1213–1219 (2019). https://doi.org/10.1007/s10482-019-01253-z

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Keywords

  • Pseudoxanthomonas composti
  • Novel species
  • Polyphasic taxonomy