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Chitinophaga aurantiaca sp. nov., isolated from a soil sample from a tangerine field

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Abstract

A Gram-stain-negative, facultatively anaerobic, non-motile and rod-shaped bacterial strain, designated THG-SD5.5T, was isolated from a soil sample collected in a tangerine field, Republic of Korea. According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Chitinophaga and to be closely related to Chitinophaga ginsengihumi KACC 17604T (97.9%) and Chitinophaga rupis KACC 14521T (97.5%). The 16S rRNA gene sequence similarities with other species of the genus Chitinophaga were in the range 92.8–95.5%. Catalase test was positive. Oxidase test was negative. The DNA G + C content was determined to be 46.1 mol%. DNA-DNA hybridization values between strain THG-SD5.5T and C. ginsengihumi KACC 17604T and C. rupis KACC 14521T were 45.1% and 15.6%, respectively. Strain THG-SD5.5T was also found to be able to grow at 24–33 °C, at 0–5% NaCl and at pH 5.5–9.0. The major fatty acids were identified as anteiso-C15:0, C16:0, anteiso-C17:0 and C18:0. The dominant respiratory quinone was identified as menaquinone-7 (MK-7). The polar lipids were found to be diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminolipid, two unidentified phospholipids and three unidentified lipids. Based on these phenotypic, genotypic and phylogenetic characterisations, strain THG-SD5.5T (= KACC 19338T = CGMCC 1.16304T) is concluded to represent a novel species of the genus Chitinophaga, for which the name Chitinophaga aurantiaca sp. nov. is proposed.

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Funding

This work was carried out with the support of “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ012655042018)” Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdae-ro, Giheung-gu, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea

    Su-Kyung Kim, Zheng-Fei Yan, Huan Trinh & Tae-Hoo Yi

  2. Department of Food and Nutrition, Baewha Women’s University, Seoul, 03039, Republic of Korea

    MooChang Kook

  3. Seoul Hightech Venture Center, SD Biotechnologies Co., Ltd., 29, Gonghang-daero 61-gil, Gangseo-gu, Seoul, 07563, Republic of Korea

    Sang-Yong Park, Kyung Hwa Won & Jung-Eun Yang

  4. Citrus Research Institute, National Institute of Horticultural & Herbal Science, RDA, Jeju, 63607, Korea

    Sang Suk Kim

  5. Woongjin Foods R&D Center, Room 201, Jei-Platz, 459-11, GaSan-Dong, GeumCheon-Gu, Seoul, 153-792, Korea

    Ho Bin Kim

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  1. Su-Kyung Kim
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Correspondence to Tae-Hoo Yi.

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This is the original work of the authors. The work described has not been submitted elsewhere for publication, in whole or in part, and all authors listed carry out the data analysis and manuscript writing and “This article does not contain any studies with human participants or animals performed by any of the authors”. Moreover, all authors read and approved the final manuscript.

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Su-Kyung Kim and MooChang Kook are co-first authors.

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Kim, SK., Kook, M., Yan, ZF. et al. Chitinophaga aurantiaca sp. nov., isolated from a soil sample from a tangerine field. Antonie van Leeuwenhoek 112, 1189–1197 (2019). https://doi.org/10.1007/s10482-019-01251-1

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