Whole genome sequencing of a ST2594 MRSA strain causing non-mucosal preoperative colonization and low-grade postoperative infection

Abstract

We present a post-operative infection caused by a methicillin-resistant Staphylococcus aureus strain, previously isolated in the preoperative screening, in a patient submitted to femoral osteosynthesis, successfully treated with oral ciprofloxacin. The isolate exhibited in vitro resistance to ciprofloxacin, Staphylococcal Cassette Chromosome mec type IV, it was negative for the lukS-PV Panton–Valentine leucocidin gene and belonged to ST2594 in multilocus sequence typing analysis. Whole genome sequencing revealed a genome size of 2,818,289 base pairs. The annotated genomes of ST2594 and N315 strains were compared, looking for genes related to virulence and resistance. The lack of the tst, sec, sel genes, associated with a mutation in the clfA gene, may partially explain the low morbity in this case.

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References

  1. Aamot HV et al (2012) Molecular characterisation of methicillin-sensitive Staphylococcus aureus from deep surgical site infections in orthopaedic patients. Eur J Clin Microbiol Infect Dis 31(8):1999–2004

    Article  CAS  PubMed  Google Scholar 

  2. Al-Talib H et al (2009) A pentaplex PCR assay for the rapid detection of methicillin-resistant Staphylococcus aureus and Panton–Valentine Leucocidin. BMC Microbiol 9(1): 113. http://www.biomedcentral.com/1471-2180/9/113

  3. Anderson AS et al (2016) Staphylococcus aureus clumping factor a remains a viable vaccine target for prevention of S. Aureus infect. MBio 7(2):e00225–e00316

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Boye K et al (2007) A new multiplex PCR for easy screening of methicillin-resistant Staphylococcus aureus SCC mec types I-V. Clin Microbiol Infect 13(7):725–727

    Article  CAS  PubMed  Google Scholar 

  5. Claes J et al (2017) Clumping factor A, von Willebrand factor-binding protein and von Willebrand factor anchor Staphylococcus aureus to the vessel wall. J Thromb Haemost 15(5):1009–1019

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Darling ACE et al (2004) Mauve: multiple alignment of conserved genomic sequence with rearrangements mauve: multiple alignment of conserved genomic sequence with rearrangements. Genome Res 14:1394–1403

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Dastgheyb S et al (2015) Effect of biofilms on recalcitrance of Staphylococcal joint infection to antibiotic treatment. J Infect Dis 211:641–650

    Article  CAS  PubMed  Google Scholar 

  8. EUCAST (2017) The european committee on antimicrobial susceptibility testing. Clinical breakpoints (Bacterial v7.1). http://www.eucast.org/clinical_breakpoints. Accessed 16 Aug 2017

  9. Higgins J et al (2006) Clumping factor A of Staphylococcus aureus inhibits phagocytosis by human polymorphonuclear leucocytes. FEMS Microbiol Lett 258(2):290–296

    Article  CAS  PubMed  Google Scholar 

  10. Josefsson E et al (2008) In vivo sortase A and clumping factor A mRNA expression during Staphylococcus aureus infection. Microb Pathog 44(2):103–110

    Article  CAS  PubMed  Google Scholar 

  11. Pires FV et al 2014 Nasal Carriage of Staphylococcus aureus in Botucatu, Brazil: a population-based survey. PLoS ONE, 9(3), p.e92537. Available at: http://dx.plos.org/10.1371/journal.pone.0092537

  12. Ryu S et al (2014) Colonization and infection of the skin by S. aureus: immune system evasion and the response to cationic Antimicrobial peptides. Int J Mol Sci 15(5):8753–8772

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Vergara A et al (2017) Biofilm formation and its relationship with the molecular characteristics of food-related Methicillin-resistant Staphylococcus aureus (MRSA). J Food Sci 82(10):2364–2370

    Article  CAS  PubMed  Google Scholar 

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Funding

Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq.).

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Correspondence to Robson Souza Leão.

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The authors declare that they have no conflict of interest.

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All procedures performed in this study were in accordance with the ethical standards of the institutional research committee (CAAE: 39070314.0.0000.5256).

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Informed consent was obtained from the participant included in the study.

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Bastos, L.R., Martins, M.C.F., Albano, R.M. et al. Whole genome sequencing of a ST2594 MRSA strain causing non-mucosal preoperative colonization and low-grade postoperative infection. Antonie van Leeuwenhoek 112, 961–964 (2019). https://doi.org/10.1007/s10482-019-01229-z

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Keywords

  • Bacterial genome
  • Carrier state
  • Methicillin-resistant Staphylococcus aureus
  • Surgical wound infection