Phyllobacterium phragmitis sp. nov., an endophytic bacterium isolated from Phragmites australis rhizome in Kumtag Desert

Abstract

A Gram-negative rod, designated strain 1N-3T, was isolated from a rhizome of Phragmites australis grown in Kumtag Desert, China. Phylogenetic analysis showed that the strain is closely related to Phyllobacterium salinisoli LMG 30173T with 99.0% sequence similarity in the 16S rRNA gene and 92.9% in the atpD gene. Growth was observed at salinities of 0–4% (w/v), over a pH range of 5.0–10.0 (optimum 8.0) and at temperatures of 15–40 °C (optimum 30 °C). The predominant cellular fatty acids were identified as summed feature 8 (C18:1ω7c/C18:1ω6c). The G+C content of strain 1N-3T was determined to be 60.1%. Based on phenotypic, chemotaxonomic, phylogenetic properties and genomic comparison, it is concluded that strain 1N-3T represents a novel species of the genus Phyllobacterium, for which the name Phyllobacterium phragmitis sp. nov. is proposed. The type strain is 1N-3T (=KCTC 62183T =ACCC 60071T).

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Funding

This work was supported by The State Key Research and Development Program of China (2016YFC0500801).

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Correspondence to Lei Liu.

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The authors declare that there are no conflicts of interest.

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No specific ethical or institutional permits were required to conduct sampling and the experimental studies did not involve endangered or protected species.

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Liang, Lx., Sun, Qw., Hui, N. et al. Phyllobacterium phragmitis sp. nov., an endophytic bacterium isolated from Phragmites australis rhizome in Kumtag Desert. Antonie van Leeuwenhoek 112, 661–668 (2019). https://doi.org/10.1007/s10482-018-1195-5

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Keywords

  • Phragmites australis
  • Phyllobacterium
  • Novel species
  • Rhizome