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Georgenia alba sp. nov., a novel halotolerant actinobacterium isolated from a desert sand sample

Antonie van Leeuwenhoek volume 112, pages 203–209 (2019)Cite this article

Abstract

Strain SYSU D8008T was isolated from a desert sand sample collected from Saudi Arabia. The strain was observed to be Gram-stain positive, non-motile and aerobic. It can grow at 15–37 °C, pH 6.0–10.0 and can tolerant up to 7% (w/v) NaCl. Pairwise comparison of the 16S rRNA gene sequence indicated that strain SYSU D8008T shares high sequence similarities with Georgenia deserti SYSU D8004T (96.8%) and Georgenia halophila YIM 93316T (96.8%). Menaquinone MK-8(H4) was detected as the respiratory isoprenologue. The polar lipid profile of strain SYSU D8008T was found to consist of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two phosphatidylinositol mannosides, an unidentified glycolipid and an unidentified phospholipid. Strain SYSU D8008T was found to contain anteiso-C15:0 and iso-C15:0 as the predominant fatty acids. Galactose, glucose and rhamnose were detected as the whole cell sugars. Based on the phenotypic, genotypic and phylogenetic characteristics, strain SYSU D8008T can be differentiated from other closely related members of the genus Georgenia. The strain SYSU D8008T, therefore, is concluded to represent a novel species of the genus Georgenia, for which the name Georgenia alba sp. nov. is proposed. The type strain is SYSU D8008T (= CGMCC 1.15800T = KCTC 39988T).

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Acknowledgements

This research was supported by the Xinjiang Uygur Autonomous Region regional coordinated innovation project (Shanghai Cooperation Organization Science and Technology Partnership Program) (Grant No. 2017E01031), the Deanship of Scientific Research at Princess Nourah bint Abdulrahman University, through the Research Groups Program (Grant No. RGP-1438-0004), National Natural Science Foundation of China (Grant No. 31850410475) and China Biodiversity Observation Networks (SinoBON). WJL is supported by project funded by Guangdong Province Higher Vocational Colleges and Schools Pearl River Scholar Funded Scheme (2014).

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Author notes

  1. Lan-Yu Li and Zi-Wen Yang have contributed equally to this work.

Authors and Affiliations

  1. College of Life Sciences, Henan Normal University, Xinxiang, 453007, People’s Republic of China

    Lan-Yu Li, Guo-Xing Nie & Wen-Jun Li

  2. State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

    Zi-Wen Yang, Mipeshwaree Devi Asem, Nimaichand Salam, Min Xiao & Wen-Jun Li

  3. Biology Department, Faculty of Science, Princess Nourah Bint Abdulrahman University, Riyadh, 11564, Kingdom of Saudi Arabia

    Dalal Hussien M. Alkhalifah

  4. Bioproducts Research Chair, Zoology Department, College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia

    Wael N. Hozzein

  5. Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi, 830011, People’s Republic of China

    Wen-Jun Li

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  1. Lan-Yu Li
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Contributions

N.S. and W.J.L. conceived the study. L.Y.L., Z.W.Y., M.D.A. and M.X. performed research. D.H.M.A., W.N.H and N.S. analyzed data. Z.W.Y., N.S. and W.J.L. wrote the paper. All authors approved the manuscript.

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Correspondence to Nimaichand Salam or Wen-Jun Li.

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Li, LY., Yang, ZW., Asem, M.D. et al. Georgenia alba sp. nov., a novel halotolerant actinobacterium isolated from a desert sand sample. Antonie van Leeuwenhoek 112, 203–209 (2019). https://doi.org/10.1007/s10482-018-1145-2

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Keywords

  • Georgenia alba sp. nov.
  • Saudi Arabia
  • Desert
  • Polyphasic taxonomy