Anoxybacillus sediminis sp. nov., a novel moderately thermophilic bacterium isolated from a hot spring

Abstract

A Gram-stain positive, moderately thermophilic, aerobic, spore-forming and rod-shaped bacterium, designated YIM 73012T, was isolated from a sediment sample collected from a hot spring located in Tibet, China, and was characterized by using a polyphasic taxonomy approach. The strain is oxidase positive and catalase negative. Growth occurred at 37–65 °C (optimum, 45–50 °C), at pH 6.0–8.5 (optimum, pH 7.0–7.5) and with 0.5–3.5% NaCl (optimum, 0.5–1.0%, w/v). The major fatty acids were iso-C15:0, iso-C16:0 and C16:0. The major polar lipids comprised of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine and phosphatidylglycerol. The cell wall peptidoglycan contained meso-diaminopimelic acid. The respiratory quinone was MK-7. The G+C content of genomic DNA was 43.6 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain YIM 73012T forms a distinct lineage with respect to the genus Anoxybacillus in the family Bacillaceae. Based on 16S rRNA gene sequence identities the closely related phylogenetic neighbours are Anoxybacillus caldiproteolyticus DSM 15730T (96.7%) and Saccharococcus thermophilus DSM 4749T (96.6%). Strain YIM 73012T was distinguishable from the closely related reference strains by the differences in phenotypic, chemotaxonomic and genotypic characteristics, and represents a novel species of the genus Anoxybacillus, for which the name Anoxybacillus sp. nov. is proposed. The type species is Anoxybacillus sediminis sp. nov., with the type strain YIM 73012T (= KCTC 33884T = DSM 103835T).

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Acknowledgements

The authors are grateful to Dr. Rüdiger Pukall (DSMZ, Germany) for providing the reference type strains. This work was supported by the Key Project of International Cooperation of Ministry of Science and Technology (MOST, China) (No. 2013DFA31980), Science and Technology Infrastructure work project (No. 2015FY110100), National Natural Science Foundation of China (No. 31470139) and Basic Scientific Research Service Fee Project in Colleges and Universities (No. 17lgjc19). W.-J. Li was also supported by Guangdong Province Higher Vocational Colleges and Schools Pearl River Scholar Funded Scheme (2014).

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IUK, NH and. W-JL conducted this study. IUK, NH, AMD and. MX performed the experiments. XYZ and W-JL supervised the experiments. IUK, MSH, MH and NS wrote the manuscript. All of the authors assisted in writing the manuscript, discussed the results and commented on the manuscript

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Correspondence to Xiao-Yang Zhi or Wen-Jun Li.

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Khan, I.U., Habib, N., Xiao, M. et al. Anoxybacillus sediminis sp. nov., a novel moderately thermophilic bacterium isolated from a hot spring. Antonie van Leeuwenhoek 111, 2275–2282 (2018). https://doi.org/10.1007/s10482-018-1118-5

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Keywords

  • Anoxybacillus sediminis sp. nov.
  • Polyphasic taxonomy
  • Hot spring in Tibet
  • China