A strictly aerobic, Gram-stain negative, long rod-shaped, motile by gliding and yellow pigmented bacterium, designated strain w01T, was isolated from marine sediment. The strain was characterised to determine its taxonomic position by using a polyphasic approach. Strain w01T was observed to grow optimally in the presence of 3.0% (w/v) NaCl, at 30 °C and to hydrolyse Tweens 20, 40 and 80, starch, casein and alginate. Carotenoid pigments were found to be produced but not flexirubin-type pigments. On the basis of 16S rRNA gene sequence similarities, strain w01T is phylogenetically affiliated with the genus Aquimarina and is closely related to Aquimarina macrocephali JCM 15542T (97.4% sequence similarity) and Aquimarina muelleri KCTC 12285T (97.0%). Lower sequence similarities (< 97.0%) were found with the other currently recognised members of the genus Aquimarina. The predominant fatty acids were identified as iso-C15:0 (33.7%), C18:0 3-OH (16.8%) and C17:1ω7c (10.6%). The polar lipid profile was found to contain phosphatidylethanolamine, an unidentified aminolipid and two unidentified polar lipids. MK-6 was identified as the sole respiratory quinone. The G + C content of the genomic DNA was determined to be 33.3 mol%. Strain w01T can be differentiated genotypically and phenotypically from recognised species of the genus Aquimarina. The isolate is therefore concluded to represent a novel species, for which the name Aquimarina sediminis sp. nov. is proposed, with the type strain w01T (= KCTC 62350T = MCCC 1H00287T).
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
Tax calculation will be finalised during checkout.
Bernadet J, Nakagawa Y, Holmes B (2002) Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and Jean-Franc by the members of the Subcommittee on the taxonomy of Flavobacterium and Cytophaga -like bacteria of the International Committee on Systematics of Prokaryotes. Int J Syst Evol Microbiol 52:1049–1070
Biebl H, Pukall R, Lunsdorf H, Schulz S, Allgaier M, Tindall BJ, Wagner Dobler I (2007) Description of Labrenzia alexandrii gen. nov., sp. nov., a novel alphaproteobacterium containing bacteriochlorophyll a, and a proposal for reclassification of Stappia aggregata as Labrenzia aggregata comb. nov., of Stappia marina as Labrenzia marina comb. Int J Syst Evol Microbiol 57:1095–1107
Bligh EG, Dyer WJ (1959) A rapid method of total lipidextraction and purification. Can J Biochem Physiol 37:911–917
Bowman JP (2000) Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868
Chen WM, Sheu FS, Sheu SY (2012) Aquimarina salinaria sp. nov., a novel algicidal bacterium isolated from a saltpan. Arch Microbiol 194:103–112
Cowan ST, Steel KJ (1965) Manual for the identification of medical bacteria. Cambridge University Press, London
Dong XZ, Cai MY (eds) (2001) Chapter 14. Determination of biochemical characteristics. In: Manual for the systematic identification of general bacteria. Science Press, Beijing, pp 370–398
Du ZJ, Wang Y, Dunlap C, Rooney AP, Chen GJ (2014) Draconibacterium orientale gen. nov., sp. nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov. Int J Syst Evol Microbiol 64:1690–1696
Fang DB, Han JR, Liu Y, Du ZJ (2017) Seonamhaeicola marinus sp. nov., isolated from marine algae. Int J Syst Evol Microbiol 67:4857–4861
Felsenstein J (2005) PHYLIP (Phylogeny Inference Package) version 3.6. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle
Jukes TH, Cantor CR (1969) Evolution of the protein molecules. In: Munro HN (ed) Mammalian protein metabolism. Academic Press, New York, pp 21–132
Kennedy J, Margassery LM, O’Leary ND, O’Gara F, Morrissey J, Dobson ADW (2014) Aquimarina amphilecti sp. nov., isolated from the sponge Amphilectus fucorum. Int J Syst Evol Microbiol 64:501–505
Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721
Lee JK, Cha IT, Kim M, Choi BG, Song HS, Kim JY, Roh SW, Lee SJ (2017) Aquimarina versatilis sp. nov., isolated from seashore sand, and emended description of the genus Aquimarina. Int J Syst Evol Microbiol 67:411–416
Li X, Wang L, Huang H, Lai Q, Shao Z (2014) Aquimarina penaei sp. nov., isolated from intestinal tract contents of Pacific white shrimp, Penaeus vannamei. Antonie Van Leeuwenhoek 106:1223–1229
Liu QQ, Wang Y, Li J, Du ZJ, Chen GJ (2014) Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans. Int J Syst Evol Microbiol 64:2204–2209
Ludwig W, Strunk O, Westram R, Richter L, Meier H, Yad-hukumar Buchner A, Lai T, Steppi S et al (2004) ARB: a software environment for sequence data. Nucleic Acids Res 32:1363–1371
Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241
Miyazaki M, Nagano Y, Fujiwara Y, Hatada Y, Nogi Y (2010) Aquimarina macrocephali sp. nov., isolated from sediment adjacent to sperm whale carcasses. Int J Syst Evol Microbiol 60:2298–2302
Nedashkovskaya OI, Kim Seung B, Lysenko AM, Frolova GM, Mikhailov VV, Lee KH, Bae KS (2005) Description of Aquimarina muelleri gen. nov., sp. nov., and proposal of the reclassification of [Cytophaga] latercula Lewin 1969 as Stanierella latercula gen. nov., comb. nov. Int J Syst Evol Microbiol 55:225–229
Nedashkovskaya OI, Vancanneyt M, Christiaens L, Kali- novskaya NI, Mikhailov VV, Swings J (2006) Aquimarina intermedia sp. nov., reclassification of Stanierella latercula (Lewin 1969) as Aquimarina latercula comb. nov. and Gaetbulimicrobium brevivitae Yoon et al. 2006 as Aquimarina brevivitae comb. nov. and emended description of the genus Aquimarina. Int J Syst Evol Microbiol 56:2037–2041
Obataa O, Akunnab JC, Walkera G (2015) Hydrolytic effects of acid and enzymatic pre-treatment on the anaerobic biodegradability of Ascophyllum nodosum and Laminaria digitata species of brown seaweed. Biomass Biogenergy 80:140–146
Oh Y, Choi B, Kim JY, Roh SW, Lee S (2017) Aquimarina seongsanensis sp. nov., isolated from sea water. Antonie Van Leeuwenhoek 110:1019–1025
Pruesse E, Peplies J, Glöckner FO (2012) SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 28:1823–1829
Reichenbach H (1992) Validation of the publication of new names and new combinations previously effectively published outside the IJSBi: list No. 41. Int J Syst Evol Microbiol 42:327–328
Richter M, Rossello-Mora R (2009) Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 106:19126–19131
Rosselló-Móra R, Trujillo ME, Sutcliffe IC (2017) Introducing a digital protologue: a timely move towards a database-driven systematics of Archaea and Bacteria. Syst Appl Microbiol 40:121–122
Smibert RM, Krieg NR (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general, molecular bacteriology. American Society for Microbiology, Washington, pp 607–654
Stamatakis A (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30:1312–1313
Takeshita S, Oda T, Muramatsu T (1991) An improved plate method, in the presence of calcium chloride or sulfuric acid, for simultaneous detection of alginate lyases. Agric Biol Chem 55:2637–2638
Tindall BJ (1990a) A Comparative Study of the Lipid Composition of Halobacterium saccharovorum from Various Sources. Syst Appl Microbiol 13:128–130
Tindall BJ (1990b) Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66:199–202
Yarza P, Richter M, Peplies J, Euzeby J, Amann R, Schleifer KH, Ludwig W, Glöckner FO, Rosselló-Móra R (2008) The all-species living tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. Syst Appl Microbiol 31:241–250
Yu T, Zhang Z, Fan X, Shi X, Zhang XH (2014) Aquimarina megaterium sp. nov., isolated from seawater. Int J Syst Evol Microbiol 64:122–127
Zhou YX, Wang C, Du ZJ, Chen GJ (2015) Aquimarina agarivorans sp. nov., a genome-sequenced member of the class Flavobacteriia isolated from Gelidium amansii. Int J Syst Evol Microbiol 65:2684–2688
This work was supported by the National Natural Science Foundation of China (31770002).
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with animals performed by any of the authors.
Electronic supplementary material
Below is the link to the electronic supplementary material.
About this article
Cite this article
Wang, NN., Zhou, LY., Li, YX. et al. Aquimarina sediminis sp. nov., isolated from coastal sediment. Antonie van Leeuwenhoek 111, 2257–2265 (2018). https://doi.org/10.1007/s10482-018-1115-8
- 16S rRNA gene
- Polyphasic taxonomy