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Flavobacterium zaozhuangense sp. nov., a new member of the family Flavobacteriaceae, isolated from metolachlor-contaminated soil

Antonie van Leeuwenhoek Aims and scope Submit manuscript

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Abstract

Strain ZZ-8T, a Gram-negative, aerobic, non-spore-forming, non-motile, yellow-pigmented, rod-shaped bacterium, was isolated from metolachlor-contaminated soil in China. The taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain ZZ-8T is a member of the genus Flavobacterium and shows high sequence similarity to Flavobacterium humicola UCM-46T (97.2%) and Flavobacterium pedocola UCM-R36T (97.1%), and lower (< 97%) sequence similarity to other known Flavobacterium species. Chemotaxonomic analysis revealed that strain ZZ-8T possessed MK-6 as the major respiratory quinone; and iso-C15:0 (28.5%), summed feature 9 (iso-C17:1 w9c/C16:0 10-methyl, 22.9%), iso-C17:0 3-OH (17.0%), iso-C15:0 3-OH (8.9%), iso-C15:1 G (8.6%) and summed feature 3 (C16:1 w7c/C16:1 w6c, 5.7%) as the predominant fatty acids. The polar lipids of strain ZZ-8T were determined to be lipids, a glycolipid, aminolipids and phosphatidylethanolamine. Strain ZZ-8T showed low DNA–DNA relatedness with F. pedocola UCM-R36T (43.23 ± 4.1%) and F. humicola UCM-46T (29.17 ± 3.8%). The DNA G+C content was 43.3 mol%. Based on the phylogenetic and phenotypic characteristics, chemotaxonomic data and DNA–DNA hybridization, strain ZZ-8T is considered a novel species of the genus Flavobacterium, for which the name Flavobacterium zaozhuangense sp. nov. (type strain ZZ-8T = KCTC 62315 T = CCTCC AB 2017243T) is proposed.

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Abbreviations

KCTC:

Korean collection for type cultures

CCTCC:

China Center for type culture collection

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Acknowledgements

This work was financially supported through grants from the National Natural Science Foundation of China (31600080) and the Natural Science Foundation of Shandong Province, China (ZR2016CB29 and ZR2017QD012).

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Author notes

  1. Qing Chen and Hong-Mei Wang have contributed equally to this work.

Authors and Affiliations

  1. College of Life Sciences, Zaozhuang University, Zaozhuang, 277160, Shandong, China

    Qing Chen, Hong-Mei Wang, Zhong-Guan Sun, Jing Chen & Yan Ge

  2. College of City and Architecture Engineering, Zaozhuang University, Zaozhuang, 277160, Shandong, China

    Wen Zhuang

  3. Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China

    Ji-guo Qiu & Jian He

Authors
  1. Qing Chen
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  2. Hong-Mei Wang
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  3. Wen Zhuang
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  4. Zhong-Guan Sun
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  5. Jing Chen
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  7. Ji-guo Qiu
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Correspondence to Ji-guo Qiu.

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Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain ZZ-8T is MG712774.

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Chen, Q., Wang, HM., Zhuang, W. et al. Flavobacterium zaozhuangense sp. nov., a new member of the family Flavobacteriaceae, isolated from metolachlor-contaminated soil. Antonie van Leeuwenhoek 111, 1977–1984 (2018). https://doi.org/10.1007/s10482-018-1090-0

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