Acidovorax monticola sp. nov., isolated from soil
A novel strain K-4-16T was isolated from forest soil of Namsan Mountain, Seoul, South Korea, and was taxonomically characterized by a polyphasic approach. Strain K-4-16T was observed to be a Gram-staining negative, grayish white-coloured, motile with peritrichous flagella, and rod shaped bacterium. It was able to grow at 15–45 °C, at pH 4.5–10.5, and at 0–4% (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, strain K-4-16T belongs to the genus Acidovorax and is closely related to Acidovorax anthurii CFBP 3232T (98.3% sequence identity), Acidovorax konjaci K2T (97.9% sequence identity), Acidovorax valerianellae CFBP 4730T (97.8% sequence identity), and Acidovorax caeni R-24608T (97.8% sequence identity). The only respiratory quinone was ubiquinone-8. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. The predominant fatty acids of strain K-4-16T were summed feature 3 (C16:1ω7c and/or C16:1ω6c), C16:0, and summed feature 8 (C18:1ω7c and/or C18:1ω6c). The genomic DNA G+C content of this novel strain was 64.7 mol%. The DNA–DNA relatedness between strain K-4-16T and its reference strains were below the threshold value of 70%. The morphological, physiological, chemotaxonomic, and phylogenetic analyses clearly distinguished this strain from its close phylogenetic neighbors. Thus, strain K-4-16T represents a novel species of the genus Acidovorax, for which the name Acidovorax monticola sp. nov. is proposed. The type strain is K-4-16T (= KEMB 9005-570T = KACC 19171T = NBRC 113141T).
KeywordsAcidovorax monticola Mountain soi Comamonadaceae Proteobacteria Novel species
This work was supported by a Kyonggi University Research Grant (2017-011).
Compliance with ethical standards
Conflict of interest
The authors declare that there is no conflict of interest.
This study does not describe any experimental work related to humans.
- Beveridge TJ, Lawrence JR, Murray RGE (2007) Sampling and staining for light microscopy. In: Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM, Snyder LR (eds) Methods for general and molecular microbiology, 3rd edn. American Society for Microbiology, Washington, DC, pp 19–33Google Scholar
- Breznak JA, Costilow RN (2007) Physicochemical factors in growth. In: Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM, Snyder LR (eds) Methods for general and molecular microbiology, 3rd edn. American Society for Microbiology, Washington, DC, pp 309–329Google Scholar
- Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229CrossRefGoogle Scholar
- Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
- Hemraj V, Diksha S, Avneet G (2013) A review on commonly used biochemical test for bacteria. Innovare J Life Sci 1:1–7Google Scholar
- Saitou N, Nei M (1987) The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425Google Scholar
- Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. MIDI Inc., NewarkGoogle Scholar
- Smibert RM, Krieg NR (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC, pp 607–654Google Scholar
- Tindall BJ, Sikorski J, Smibert RA, Krieg NR (2007) Phenotypic characterization and the principles of comparative systematics. In: Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM, Snyder LR (eds) Methods for general and molecular microbiology, 3rd edn. American Society for Microbiology, Washington, DC, pp 330–393Google Scholar
- Willems A, Falsen E, Pot B, Jantzen E, Hoste B, Vandamme P, Gillis M, Kersters K, De Ley J (1990) Acidovorax, a new genus for Pseudomonas facilis, Pseudomonas delafieldii, E. Falsen (EF) group 13, EF group 16, and several clinical isolates, with the species Acidovorax facilis comb. nov., Acidovorax delafieldii comb. nov., and Acidovorax temperans sp. nov. Int J Syst Evol Microbiol 40:384–398Google Scholar
- Willems A, Goor M, Thielemans S, Gillis M, Kersters K, De Ley J (1992) Transfer of several phytopathogenic Pseudomonas species to Acidovorax as Acidovorax avenae subsp. avenae subsp. nov., comb. nov., Acidovorax avenae subsp. citrulli, Acidovorax avenae subsp. cattleyae, and Acidovorax konjaci. Int J Syst Evol Microbiol 42:107–119Google Scholar