Hoeflea prorocentri sp. nov., isolated from a culture of the marine dinoflagellate Prorocentrum mexicanum PM01

Abstract

A Gram-stain negative, aerobic, rod-shaped, non-motile, yellow-pigmented and non-spore-forming bacterial strain, designated PM5-8T, was isolated from a culture of a marine toxigenic dinoflagellate Prorocentrum mexicanum PM01. Strain PM5-8T grew at 15–35 °C (optimum, 25–30 °C) and pH 6–11 (optimum, 7.5–8). Cells required at least 1.5% (w/v) NaCl for growth, and can tolerate up to 7.0% with the optimum of 4%. Phylogenetic analysis based on 16S rRNA gene sequence revealed that the strain PM5-8T is closely related to members of the genus Hoeflea, with high sequence similarities with Hoeflea halophila JG120-1T (97.06%) and Hoeflea alexandrii AM1V30T (97.01%). DNA–DNA hybridization values between the isolate and other type strains of recognized species of the genus Hoeflea were between 11.8 and 25.2%, which is far below the value of 70% threshold for species delineation. The DNA G + C content was 50.3 mol%. The predominant cellular fatty acids of the strain were identified as summed feature 8 (C16:1 ω7c and/or C16:1 ω6c; 51.5%), C18:1 ω7c 11-methyl (20.7%), C16:0 (17.2%) and C18:0 (5.7%). The major respiratory quinone was Q-10. Polar lipids profiles contained phosphatidylcholine, phosphatidylglycerol, sulfoquinovosyl diacylglycerol, phosphatidylmono- methylethanolamine, phosphatidylethanolamine and four unidentified lipids. On the basis of the polyphasic taxonomic data presented, strain PM5-8T (= CCTCC AB 2016294T = KCTC 62490T) represents a novel species of the genus Hoeflea, for which the name Hoeflea prorocentri sp. nov. is proposed.

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Acknowledgements

This work was supported by Talent Introduction Foundation of Zhejiang Ocean University (for Qiao Y.), the National Natural Science Foundation of China (41206093 and 31470540), the Zhejiang Provincial Natural Science Foundation of China (LY18D060007), Scientific Instrument and Chemical Reagents Project of Shanghai Science and Technology Committee (15142201600), and Municipal Public Welfare Project of Zhoushan (2017C32083). The authors also sincerely thank Prof. Hong-Nong Chou at Institute of Fisheries Science, National Taiwan University, for kindly providing Prorocentrum mexicanum PM01.

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Correspondence to Xiao-Ling Zhang or Jun Mu.

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The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence of strain PM5-8T is KY264918.

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Yang, Q., Jiang, ZW., Huang, CH. et al. Hoeflea prorocentri sp. nov., isolated from a culture of the marine dinoflagellate Prorocentrum mexicanum PM01. Antonie van Leeuwenhoek 111, 1845–1853 (2018). https://doi.org/10.1007/s10482-018-1074-0

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Keywords

  • Hoeflea prorocentri sp. nov.
  • Novel species
  • Prorocentrum mexicanum
  • Algal-associated bacterium