Antonie van Leeuwenhoek

, Volume 111, Issue 10, pp 1825–1833 | Cite as

A novel pigmented and heavy metal biosorptive bacterium, Leucobacter epilobiisoli sp. nov., isolated from rhizosphere soil of Epilobium hirsutum L.

  • Shao-Yang Hou
  • Fang Wang
  • Meng-Yue Zhang
  • Juan Cheng
  • Yi-Xuan Zhang
Original Paper


A novel yellow pigmented, Gram-positive, aerobic and heavy metal biosorptive bacterium designated SYP-B2667T was isolated from rhizosphere soil of Epilobium hirsutum L. in Tongren, Guizhou province, China. Based on 16S rRNA gene sequence analyses, it was shown that strain SYP-B2667T represents a novel species in the genus Leucobacter, with Leucobacter chromiireducens subsp. solipictus JCM 15573T as a close phylogenetic neighbour (sequence similarity of 98.2%). Chemotaxonomic characteristics also supported the affiliation to the genus Leucobacter. Strain SYP-B2667T was determined to have a DNA G+C content of 66.6 mol%; 2,4-diaminobutyric acid in the cell wall peptidoglycan amino acids; MK-11 as predominant menaquinone; an abundance of anteiso-C15:0 and anteiso-C17:0 fatty acids; and polar lipids including diphosphatidylglycerol, phosphatidylglycerol, glycolipids and unidentified phospholipids. The DNA–DNA hybridization value between strain SYP-B2667T and L. chromiireducens subsp. solipictus JCM 15573T was 19.7 ± 2.8%. Based on these phylogenetic and phenotypic results, it can be concluded that strain SYP-B2667T represents a novel species, for which the name Leucobacter epilobiisoli sp. nov. is proposed. The type strain is SYP-B2667T (=DSM 105145T=CPCC 204976T). This strain can tolerate and adsorb five heavy metals and so may have potential to facilitate heavy metal removal and bioremediation.


Epilobium hirsutum L. Heavy metals biosorption Leucobacter sp. nov. Rhizosphere soil 



This research was supported by the Major Science and Technique Programs in Yunnan Province (2016ZF001-001, 2017IB038), Project of Yunnan provincial academy of science and technology (2015IC017) and National Science and Technology Major Project (2018ZX09735001-002-002).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no direct or indirect conflict of interest.

Ethical approval

This is the original work of the authors. The work described has not been submitted elsewhere for publication, in whole or in part, and all authors listed carried out the data analysis and manuscript writing. Moreover, all authors read and approved the final manuscript.

Supplementary material

10482_2018_1071_MOESM1_ESM.docx (1.1 mb)
Supplementary material 1 (DOCX 1132 kb)


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© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  1. 1.School of Life Science and BiopharmaceuticsShenyang Pharmaceutical UniversityShenyangPeople’s Republic of China

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