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Antonie van Leeuwenhoek

, Volume 111, Issue 7, pp 1165–1174 | Cite as

Corynebacterium fournierii sp. nov., isolated from the female genital tract of a patient with bacterial vaginosis

  • Khoudia Diop
  • Thi Tien Nguyen
  • Jérémy Delerce
  • Nicholas Armstrong
  • Didier Raoult
  • Florence Bretelle
  • Florence Fenollar
Original Paper

Abstract

Strain Marseille-P2948T, a novel Gram-positive, catalase-positive bacterium was isolated from a vaginal sample of a patient with bacterial vaginosis. It was characterised using the taxonogenomic approach. Phylogenetic analysis revealed that the 16S rRNA and the rpoB genes exhibit 98.7 and 93.4% similarity, respectively, with those of Corynebacterium ureicelerivorans strain IMMIB RIV-301T. Biochemical tests of strain Marseille-P2948T gave results that were similar to those of other validly named Corynebacterium species, whereas chemotaxonomic tests showed the presence of C16:0, C18:1n9, C18:0, and C18:2n6 in the fatty acid profile. The draft genome of strain Marseille-P2948T is 2,383,644 bp long in size with a G+C content of 65.03%. Of the 2210 predicted genes, 2147 are protein-coding genes and 63 are RNAs. Based on phenotypic, phylogenic and genomic results, it was concluded that the isolate represents a new species within the genus Corynebacterium. The name Corynebacterium fournierii sp. nov. is proposed and the type strain is Marseille-P2948T (= CSUR P2948 = DSM 103271).

Keywords

Corynebacterium fournierii Bacterial vaginosis Culturomics Taxogenomics Genome 

Abbreviations

CSUR

Collection de souches de l’Unité des Rickettsies

DSM

Deutsche Sammlung von Mikroorganismen

MALDI-TOF

Matrix-assisted laser-desorption/ionization time-of-flight

TE buffer

Tris-EDTA buffer

URMITE

Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes

Notes

Acknowledgements

This study was supported by the Fondation Méditerranée Infection and the French National Research Agency under the “Investissements d’avenir” program, reference ANR-10-IAHU-03. We also thank TradOnline for English reviewing and Frederic Cadoret for administrative assistance.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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Supplementary material 4 (DOCX 42 kb)

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Khoudia Diop
    • 1
  • Thi Tien Nguyen
    • 2
  • Jérémy Delerce
    • 2
  • Nicholas Armstrong
    • 2
  • Didier Raoult
    • 2
    • 3
  • Florence Bretelle
    • 2
    • 4
  • Florence Fenollar
    • 1
  1. 1.Aix Marseille Univ, IRD, AP-HM, SSA, VITROME, IHU-Méditerranée InfectionMarseilleFrance
  2. 2.Aix-Marseille Univ, IRD, AP-HM, MEPHI, IHU-Méditerranée InfectionMarseilleFrance
  3. 3.Special Infectious Agents Unit, King Fahd Medical Research CenterKing Abdulaziz UniversityJeddahSaudi Arabia
  4. 4.Department of Gynecology and Obstetrics, GynépoleHôpital Nord, AP-HMMarseilleFrance

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