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Antonie van Leeuwenhoek

, Volume 111, Issue 7, pp 1149–1156 | Cite as

Lactobacillus kosoi sp. nov., a fructophilic species isolated from kôso, a Japanese sugar-vegetable fermented beverage

  • Tai-Ying Chiou
  • Wataru Suda
  • Kenshiro Oshima
  • Masahira Hattori
  • Chiaki Matsuzaki
  • Kenji Yamamoto
  • Tomoya Takahashi
Original Paper

Abstract

A novel Gram-positive, fructophilic, catalase negative, and rod-shaped strain, designated strain 10HT was isolated from kôso, a Japanese sugar-vegetable fermented beverage obtained from a food processing factory in Saku City, Nagano Prefecture, Japan. Phylogenetic analysis based on 16S rRNA gene sequences revealed strain 10HT to belong to the genus Lactobacillus, with closely related type strains being Lactobacillus kunkeei YH-15T (95.5% sequence similarity), Lactobacillus ozensis Mizu2-1T (95.4% sequence similarity), and Lactobacillus apinorum Fhon13NT (95.3% sequence similarity). The isolate was found to grow at 18–39 °C (optimum 27 °C), pH 4.0–7.0 (optimum pH 6.5) and in the presence of 0–2% NaCl (optimum 0% NaCl). The G + C content of its genomic DNA was determined to be 30.5 mol%. The major fatty acid (≥ 10%) components identified included C16:0, C19:0 cyclo ω7c, C19:0 cyclo ω9c, and C18:1 ω9c. The polar lipids were identified as lysophosphatidylethanolamine, phosphatidylethanolamine and glycolipids. The predominant isoprenoid quinones (> 10%) were identified as MK-7, MK-8, MK-9 and MK-10. The amino acid composition of the cell wall was detected as comprising Asp, Glu, Ala, and Lys but the strain lacks meso-diaminopimelic acid. As with other fructophilic lactic acid bacteria, such as L. kunkeei and L. apinorum, strain 10HT was found to prefer d-fructose to d-glucose as a growth substrate. On the basis of these genetic and phenotypic results, the isolate is concluded to represent a novel species, for which the name Lactobacillus kosoi is proposed. The type strain is 10HT (= NBRC 113063T = BCRC 81100T).

Keywords

Lactobacillus kosoi sp. nov. Kôso Plyphasic taxonomy Fructophilic lactic acid bacteria 16S rRNA gene 

Notes

Acknowledgements

The authors are grateful to the Arsoa R&D Center and the Arsoa Saku Factory for technical supports and sample collection during this study.

Conflict of interest

The authors declare that they have no direct or indirect conflict of interest.

Supplementary material

10482_2018_1019_MOESM1_ESM.docx (866 kb)
Supplementary material 1 (DOCX 867 kb)

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Biotechnology and Environmental Chemistry, Laboratory of Food Science and TechnologyKitami Institute of TechnologyKitamiJapan
  2. 2.Department of Computational Biology, Graduate School of Frontier SciencesThe University of TokyoKashiwaJapan
  3. 3.Department of Microbiology and ImmunologyKeio University School of MedicineShinjukuJapan
  4. 4.Graduate School of Advanced Science and EngineeringWaseda UniversityShinjukuJapan
  5. 5.Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural UniversityNonoichiJapan
  6. 6.AOB Keioh Group Corporation, ARSOA Research & Development CenterHokutoJapan

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