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Pseudofrancisella aestuarii gen. nov., sp. nov., a novel member of the family Francisellaceae isolated from estuarine seawater

Abstract

A Gram-negative, aerobic, non-motile and non-spore forming bacterium, designated strain SYSU WZ-2T, was isolated from an estuarine seawater sample. Growth of strain SYSU WZ-2T was observed at temperature range of 10–40° C (optimum, 32 °C), pH range of 6–10 (optimum, pH 7–8) and in the presence of up to 5.0% NaCl (w/v). The DNA G+C content of the novel strain was determined to be 30.1% (genome). The major polar lipids were found to be diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, an unidentified aminolipid, two unidentified aminophospholipids and two unidentified phospholipids. The major fatty acids were C18:0 3-OH (27.5%), C18:1ω9c (19.3%), C16:0 (17.0%) and C14:0 (12.9%). The respiratory quinone was found to be ubiquinone Q8. Pairwise comparison of the 16S rRNA gene sequence showed that strain SYSU WZ-2T shares high identities with members of the genera Francisella (94.8–95.9%) and Allofrancisella (93.8–94.2%). The phylogenetic dendrograms based on 16S rRNA gene sequences with the members of the family Francisellaceae showed that the strain SYSU WZ-2T formed a distinct phylogenetic lineage well separated from the members of the genera Francisella and Allofrancisella. MALDI-TOF mass spectrometric analysis also depicted a different profile for strain SYSU WZ-2T compared with those of members of the genera Francisella and Allofrancisella. Based on the above results and differences in phenotypic and chemotaxonomic features, strain SYSU WZ-2T is characterized to represent a new species of a novel genus, for which the name Pseudofrancisella aestuarii gen. nov., sp. nov. is proposed (type strain SYSU WZ-2T = KCTC 52557T = CGMCC 1.13718T).

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Acknowledgements

Ping-Hua Qu was supported by Research Fund for Outstanding Young in Higher Education Institutions of Guangdong Province (2013 LYM_0015), and Science and Technology Planning Project of Guangdong Province, China (2017A0202150683). Wen-Jun Li was supported by the Guangdong Province Higher Vocational Colleges and Schools Pearl Scholar Founded Scheme (2014).

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WJL and PHQ designed research and project outline. MLZ, LD, MXH, LHL and MX performed isolation, deposition and identification. PHQ and CC performed MALDI-TOF–MS analysis. JYJ and WJL performed genome analysis. PHQ, MLZ and WJL drafted the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ping-Hua Qu or Wen-Jun Li.

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Zheng, ML., Jiao, JY., Dong, L. et al. Pseudofrancisella aestuarii gen. nov., sp. nov., a novel member of the family Francisellaceae isolated from estuarine seawater. Antonie van Leeuwenhoek 112, 877–886 (2019). https://doi.org/10.1007/s10482-018-01220-0

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Keywords

  • Pseudofrancisella aestuarii gen. nov., sp. nov.
  • Estuarine seawater
  • Polyphasic taxonomy