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Glycomyces tritici sp. nov., isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Glycomyces

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Abstract

A novel actinomycete strain, designated NEAU-C2T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.), and subjected to a polyphasic taxonomic study. Morphological and chemotaxonomic properties of this strain were consistent with the description of the genus Glycomyces. Growth was found to occur at a temperature range of 15–40 °C, pH 6–10 and NaCl concentrations of 0–4%. The cell wall was found to contain meso-diaminopimelic acid and the whole cell sugars were identified as galactose, xylose and ribose. The predominant menaquinones were identified as MK-10(H6) and MK-10(H2). The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylinositol, phosphatidylglycerol, phosphoglycolipids and an unidentified glycolipid. The major fatty acids were identified as anteiso-C15:0, iso-C16:0, anteiso-C17:0 and iso-C15:0. 16S rRNA gene sequence similarity studies showed that strain NEAU-C2T belongs to the genus Glycomyces with high sequence similarity to Glycomyces algeriensis NRRL B-16327T (99.0% sequence similarity). Some physiological and biochemical properties and low DNA–DNA relatedness values enabled the strain to be differentiated from closely related species of the genus Glycomyces. It is concluded that the isolate can be classified as representing a novel species of the genus Glycomyces, for which the name Glycomyces tritici is proposed. The type strain is NEAU-C2T (= DSM 104644T = CGMCC 4.7410T).

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Acknowledgements

This work was supported in part by Grants from the National Key Research and Development Plan (No. 2017YFD0201606), Chang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang (CSCP).

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Authors and Affiliations

  1. Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People’s Republic of China

    Wenchao Li, Chongxi Liu, Xiaowei Guo, Wei Song, Tianyu Sun, Liping Duan, Xiangjing Wang, Junwei Zhao & Wensheng Xiang

  2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China

    Wensheng Xiang

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  1. Wenchao Li
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  2. Chongxi Liu
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  3. Xiaowei Guo
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  4. Wei Song
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  5. Tianyu Sun
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  8. Junwei Zhao
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  9. Wensheng Xiang
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Correspondence to Junwei Zhao or Wensheng Xiang.

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Li, W., Liu, C., Guo, X. et al. Glycomyces tritici sp. nov., isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Glycomyces. Antonie van Leeuwenhoek 111, 1087–1093 (2018). https://doi.org/10.1007/s10482-017-1011-7

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