Ponticoccus alexandrii sp. nov., a novel bacterium isolated from the marine toxigenic dinoflagellate Alexandrium minutum
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During an investigation of the biodiversity of the cultivable bacterial community associated with paralytic shellfish poisoning toxin-producing marine dinoflagellate, Alexandrium minutum a novel algal-associated bacterium, designated strain AT2-AT was isolated. 16S rRNA gene sequence similarity analysis showed that the strain is a member of the genus Ponticoccus, with high sequence similarity to Ponticoccus litoralis DSM 18986T (97.9%) and Ponticoccus lacteus JCM 30379T (96.0%). However, based on the data obtained for the physiological and biochemical characteristics, and low level of DNA–DNA relatedness analysis, the strain could be genotypically and phenotypically differentiated from two type strains of the genus Ponticoccus. Therefore, this algal-associated bacterial strain is concluded to represent a novel species of the genus Ponticoccus, for which the name Ponticoccus alexandrii sp. nov. is proposed. The type strain is AT2-AT (CCTCC AB 2017228 T = KCTC 52626 T ).
KeywordsPonticoccus alexandrii sp. nov Marine dinoflagellate Alexandrium minutum Algal-associated bacterium
This work was supported by Talent Introduction Foundation of Zhejiang Ocean University (for Qiao Yang), the National Natural Science Foundation of China (41206093 and 31470540), the Zhejiang Provincial Natural Science Foundation of China (LY18D060007), the fund from Central Non-profit Research Institutes (East China Sea Fisheries Research Institute) (2012T08). The authors also thank Dr. Kai Tang at Xiamen University for kindly providing the type strain of Ponticoccus lacteus.
Conflict of interest
All the authors have declared no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Doetsch RN (1981) Determinative methods of light microscopy. In Manual of Methods for General Bacteriology, pp. 21–33. Edited by P. Gerdhardt, R. G. E. Murray R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg G B Phillips. Washington, DC: American Society for MicrobiologyGoogle Scholar
- Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229CrossRefGoogle Scholar
- Giovannoni S, Rappé M (2000) Evolution, diversity and molecular ecology of marine prokaryotes. In Microbial Ecology of the Oceans, pp. 47–84. Edited by D. L. Kirchman. New York: WileyGoogle Scholar
- Kates M (1972) Techniques of Lipidology: Isolation, Analysis and Identification of Lipids,: pp. 268-618. Amsterdam, ElsevierGoogle Scholar
- Lane DJ (1991) 16S–23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 125–175. Edited by E. Stackebrandt M. Goodfellow. Chichester: WileyGoogle Scholar
- Smibert RM, Krieg NR (1994) Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood N. R. Krieg. Washington, DC: American Society for MicrobiologyGoogle Scholar
- Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE, Stackebrant E, Starr HG, Tru¨per G (1987) International committee on systematic bacteriology. Report of the adhoc committee on reconciliation of approaches to bacterialsystematics. Int J Syst Bacteriol 37:463–464CrossRefGoogle Scholar
- Zhang X, Tian X, Ma L, Feng B, Liu Q, Yuan L, Yang Q (2015a) Biodiversity of the symbiotic bacteria associated with toxic marine dinoflagellates Alexandrium tamarense. J Biosci Med 3:23–28Google Scholar