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Aidingibacillus halophilus gen. nov., sp. nov., a novel member of the family Bacillaceae

Antonie van Leeuwenhoek volume 111, pages 601–608 (2018)Cite this article

Abstract

A Gram-positive, non-motile, asporogenous and aerobic bacterium, designated YIM 98012T, was isolated from a salt lake in China. Strain YIM 98012T was found to be catalase and oxidase positive. Optimal growth of strain YIM 98012T was observed at 37 °C and pH 7.0 and it was found to grow in the presence of 5–20% (w/v) NaCl (optimum 10% NaCl). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the novel strain is affiliated with the family Bacillaceae of the phylum Firmicutes and that it shares high (94.7%) sequence similarity with Alteribacillus persepolensis DSM 21632T and does not show sequence similarities of more than 94.0% to known members of other related genera. The major fatty acids (> 10%) were identified as anteiso-C15:0, anteiso-C17:0, iso-C16:0 and C16:0. The genomic DNA G+C content was determined to be 41.0 mol% and the dominated respiratory quinone was identified as MK-7. The cell wall peptidoglycan of strain YIM 98012T was found to contain meso-diaminopimelic acid, while the polar lipids profile was found to include diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine. Based on physiological and chemotaxonomic characteristics, strain YIM 98012T is concluded to be the type strain of the type species of a novel genus in the family Bacillaceae for which the name Aidingibacillus halophilus gen. nov., sp. nov. is proposed. The type strain is YIM 98012T (= KCTC 33868T = DSM 104332T).

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (No. 31400438, 31270055, 31560026).

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Author notes

  1. Yun Wang and Wei-Xun Chunyu contributed equally to this work.

Affiliations

  1. Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi, 830011, People’s Republic of China

    Yun Wang & Bo Zhang

  2. Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Yang Ji, Zhang-Gui Ding & Shu-Kun Tang

  3. Department of Pathogen Biology and Immunology, Kunming Medical University, Kunming, 650500, People’s Republic of China

    Wei-Xun Chunyu

  4. Xinjiang Entry-Exit Inspection and Quarantine Bureau, Ürümqi, 830063, People’s Republic of China

    Gang-Qiang Jiang & Ling Huang

  5. School of Medicine, Yunnan University, Kunming, 650091, People’s Republic of China

    Min Yin

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  1. Yun Wang
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Wang, Y., Chunyu, WX., Jiang, GQ. et al. Aidingibacillus halophilus gen. nov., sp. nov., a novel member of the family Bacillaceae . Antonie van Leeuwenhoek 111, 601–608 (2018). https://doi.org/10.1007/s10482-017-0980-x

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Keywords

  • Bacillaceae
  • Aidingibacillus halophilus gen. nov., sp. nov.
  • Salt lake
  • Polyphasic taxonomy