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Streptomyces sediminis sp. nov. isolated from crater lake sediment

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Abstract

A novel actinobacterial strain, MKSP12T, was isolated from coastal sediment of a crater lake in central Anatolia, Turkey. The taxonomic position of the strain was clarified using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain MKSP12T is closely related to Streptomyces specialis GW 41-1564T with 97.1% sequence similarity. The strain produces aerial hyphae that differentiate into spiral chains of smooth surfaced spores and grows over a temperature range of 20–37 °C, at pH 7–11 and in the presence of 3% (w/v) sodium chloride. The cell wall amino acid is LL-diaminopimelic acid and the whole cell sugars are glucose and ribose. The polar lipids profile consists of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, an unidentified aminophospholipid, two unidentified phospholipids, an unidentified glycophospholipid and eight unidentified glycolipids; iso-C16:0, iso-C16:1 G, anteiso-C17:0 and anteiso-C17:1 ω9c were identified as the predominant cellular fatty acids (> 10%). Based on morphological and chemotaxonomic characteristics, and phylogenetic analyses, the strain is considered to represent a novel species in the genus Streptomyces, for which the name Streptomyces sediminis sp. nov. is proposed with the type strain MKSP12T (= DSM 100692T = KCTC 39613T).

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Acknowledgements

HA was supported by the Scientific and Technological Research Council of Turkey (TÜBİTAK). IN was supported by Newcastle University postdoctoral fellowship.

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Authors and Affiliations

  1. Department of Molecular Biology and Genetics, Faculty of Science and Arts, Ondokuz Mayis University, Samsun, Turkey

    Hilal Ay

  2. Department of Biology, Faculty of Science and Arts, Ondokuz Mayis University, Samsun, Turkey

    Kamil Isik & Nevzat Sahin

  3. School of Natural and Environmental Sciences, Newcastle University, Ridley Building 2, Newcastle upon Tyne, NE1 7RU, UK

    Imen Nouioui, Maria del Carmen Montero-Calasanz & Hans-Peter Klenk

  4. Science Teaching Programme, Gazi Faculty of Education, Gazi University, 06500, Ankara, Turkey

    Demet Cetin

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  1. Hilal Ay
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10482_2017_970_MOESM1_ESM.docx

Fig. S1 Polar lipid profile of strain MKSP12T. Key: DPG, diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; AL, unidentified amino-lipid; GPL, unidentified glycophospholipid; PL1-2, unidentified phospholipids; GL 1-8, unidentified glycolipids. Supplementary material 1 (DOCX 911 kb)

10482_2017_970_MOESM2_ESM.docx

Fig. S2 Neighbour joining phylogenetic tree based on 16S rRNA gene sequences showing relationships between strain MKSP12T and the type strains of closely related Streptomyces species. Actinomadura rifamycini IFO 14183T was used as outgroup. The numbers above the branches are support values when larger than 50% from bootstrapping. The branches are scaled in terms of the expected number of substitutions per site. Supplementary material 2 (DOCX 150 kb)

10482_2017_970_MOESM3_ESM.docx

Table S1 Cellular fatty acid composition (%) of strain MKSP12T and closely related Streptomyces species grown on ISP 2 agar for 10 days at 28 °C. Supplementary material 3 (DOCX 18 kb)

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Ay, H., Nouioui, I., del Carmen Montero-Calasanz, M. et al. Streptomyces sediminis sp. nov. isolated from crater lake sediment. Antonie van Leeuwenhoek 111, 493–500 (2018). https://doi.org/10.1007/s10482-017-0970-z

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