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Antonie van Leeuwenhoek

, Volume 111, Issue 3, pp 343–351 | Cite as

Roseomonas aeriglobus sp. nov., isolated from an air-conditioning system

  • Yunho Lee
  • Che Ok Jeon
Original Paper

Abstract

A novel pale pink-coloured, strictly aerobic, Gram-stain negative bacterial strain, designated strain KER25-12T, was isolated from a laboratory air-conditioning system in South Korea. Cells were observed to be non-motile cocci showing positive catalase and oxidase reactions. Strain KER25-12T was found to grow at 10–30 °C (optimum, 25–30 °C), at pH 4.0–9.0 (optimum, pH 6.0–7.0) and in the presence of 0–2% (w/v) NaCl (optimum, 0%). Ubiquinone-10 and spermidine were detected as the sole respiratory quinone and the predominant polyamine, respectively. The major fatty acids were identified as summed feature 8 (comprising C18:1 ω7c and/or C18:1 ω6c), summed feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c), C16:0 and C18:0. The genomic DNA G+C content of strain KER25-12T was determined to be 70.0 mol%. The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and an unidentified aminolipid. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain KER25-12T belongs to the genus Roseomonas and shows high sequence similarity to Roseomonas aerilata 5420S-30T (98.57%), Roseomonas pecuniae N75T (97.44%) and Roseomonas vinacea CPCC 100056T (97.40%). Based on the morphological, physiological, chemotaxonomic and phylogenetic features, strain KER25-12T is concluded to represent a novel species of the genus Roseomonas, for which the name Roseomonas aeriglobus sp. nov. is proposed. The type strain is KER25-12T (= KACC 19282T = JCM 32049T).

Keywords

Roseomonas aeriglobus Polyphasic taxonomy Novel species Air-conditioning system 

Abbreviations

KACC

Korean Agricultural Culture Collection

JCM

Japan Collection of Microorganisms

LMG

LMG Culture Collection

DDH

DNA–DNA hybridization

Notes

Acknowledgements

This work was supported by the Program for Collection of Domestic Biological Resources from the National Institute of Biological Resources (NIBR No. 2017-02-001) of Ministry of Environment (MOE) and the National Research Foundation of Korea (2017R1A2B4004888) of MEST, Republic of Korea.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

This article does not contain any studies with human participants and/or animals performed by any of the authors. The formal consent is not required in this study.

Supplementary material

10482_2017_956_MOESM1_ESM.pdf (536 kb)
Supplementary material 1 (PDF 536 kb)

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Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  1. 1.Department of Life ScienceChung-Ang UniversitySeoulRepublic of Korea

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