Saccharopolyspora hattusasensis sp. nov., isolated from soil

Abstract

A Saccharopolyspora strain, designated CR3506T, isolated from a soil sample collected from Sungurlu, Corum, Turkey, was examinated using a polyphasic approach. Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence analysis showed that the strain is closely related to the type strains of Saccharopolyspora spinosa NRRL 18395T (99.1%), Saccharopolyspora phatthalungensis NRRL B-24798T (98.4%) and Saccharopolyspora shandongensis 88T (98.1%); low levels of DNA–DNA relatedness were found between the isolate and S. spinosa and S. phatthalungensis (<50%). Strain CR3506T was found to have chemotaxonomic and phylogenetic properties consistent with its classification in the genus Saccharopolyspora. The strain contained meso-diaminopimelic acid as the diagnostic diamino acid. Whole-cell hydrolysates contained arabinose and galactose. The polar lipids were identified as phosphatidylmethylethanolamine, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinones (>10%) were MK-9(H4) and MK-8(H4). Major fatty acids were (>10%) iso-C16:0, C15:03OH, C18:0 and iso-C15:0. Further, the morphological, physiological and biochemical characteristics of strain CR3506T are distinct from S. spinosa and other species of the genus Saccharopolyspora with which this strain has high 16S rRNA gene sequence similarity (98.0–98.5%). Strain CR3506T has antimicrobial activity against Bacillus subtilis NRRL B-209, Citrobacter freundi NRRL B-2643 and Staphylococcus aureus ATCC 29213. Consequently, it is proposed that strain CR3506T represents a novel Saccharopolyspora species for which the name Saccharopolyspora hattusasensis sp. nov. is proposed. The type strain is CR3506T (=KCTC 29104T = DSM 45715T).

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Acknowledgements

This research was supported by Ondokuz Mayis University (OMU), Project No. PYO. FEN. 1901.12.014 and Ondokuz Mayis University (OMU), Project No. PYO. FEN. 1901.09.003.

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Correspondence to Nevzat Sahin.

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Additional information

The GenBank accession number for the 16S rRNA gene sequence of Saccharopolyspora hattusasensis CR3506T (=KCTC 29104T = DSM 45715T) is JN989298.

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Supplementary Fig. S1

Scanning electron micrograph of strain CR3506T grown on ISP 3 agar at 30 °C for 21 days. Bar, 1 µm (JPEG 144 kb)

Supplementary Fig. S2

Two-dimensional TLC of polar lipids from strain CR3506T. For detection of polar lipids, molybdophosphoric acid (DPG, diphosphatidylglycerol; PME, phosphatidylmethylethanolamine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PC, phosphatidylcholine; PI, phosphatidylinositol; 6PL, six unidentified polar lipids) was applied. (JPEG 63 kb)

Supplementary Fig. S3

Maximum-likelihood tree based on almost complete 16S rRNA gene sequences showing the position of strain CR3506T with the Saccharopolyspora gene tree. Numbers at the nodes indicate levels of bootstrap support (%), only values ≥50 % are shown. Bar, 0.01 substitutions per nucleotide position. Supplementary Fig. S4. Maximum-parsimony tree based on almost complete 16S rRNA gene sequences showing the position of strain CR3506T with the Saccharopolyspora gene tree. Numbers at the nodes indicate levels of bootstrap support (%), only values ≥50 % are shown (DOC 396 kb)

Supplementary Table S1

Cellular fatty acid profiles of strain CR3506T and the type strains of related species (DOC 54 kb)

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Veyisoglu, A., Saygin, H., Tatar, D. et al. Saccharopolyspora hattusasensis sp. nov., isolated from soil. Antonie van Leeuwenhoek 110, 1719–1727 (2017). https://doi.org/10.1007/s10482-017-0921-8

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Keywords

  • Actinobacteria
  • Saccharopolyspora hattusasensis sp. nov.
  • Polyphasic taxonomy
  • 16S rRNA gene