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Nocardia tengchongensis sp. nov., isolated from a soil sample

Abstract

The taxonomic status was determined of two actinomycetes, designated CFH S0057T and CFH S0065, that were isolated from soil samples collected from an extinct volcano in Tengchong county, Yunnan province, south-west China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains CFH S0057T and CFH S0065 belong to the genus Nocardia and formed a single clade within this genus. The two isolates were able to grow at 4–45 °C, pH 5.0–7.0 and with a NaCl tolerance up to 5.0% (w/v). The whole-cell hydrolysates were rich in meso-diaminopimelic acid, galactose, arabinose and fructose. Mycolic acids were present. Strains CFH S0057T and CFH S0065 exhibited a menaquinone system with MK-8 (H4, ω cyclo), and the major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and one unidentified phospholipid. Major fatty acids were C16:0, Summed features 3, C18:1 ω9c and C18:0 10-methyl (TBSA). The genomic DNA G + C contents of strains CFH S0057T and CFH S0065 were 65.7 and 66.1 mol%, respectively. The combined genotypic, phenotypic and chemotaxonomic results indicated the isolates are considered to represent a novel species of the genus Nocardia, for which the name Nocardia tengchongensis sp. nov. is proposed. The type strain is CFH S0057T (= KCTC 29485T = JCM 30083T).

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Acknowledgements

The authors are grateful to Prof. Takuji Kudo (JCM, Japan) for his kind providing the reference type strain.

Funding information

This research was supported by Natural Science Foundation of China (No. 3150004), Program for Innovative Research Team (in Science and Technology) in University of Henan Province (14IRTSTHN013), Plan for Scientific Innovation Talent of Henan Province (154100510009), Science and technology research projects of Henan province (162102410056, 152102210292), Doctor Scientific Research Fund of Xinxiang Medical University (XYBSKYZZ201625). Ph.D. Foundation of Henan Normal University (qd13057). WJ Li was also supported by the Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme (2014).

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Correspondence to Wen-Jun Li or Guo-Xing Nie.

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Li, S., Ming, H., Duan, YY. et al. Nocardia tengchongensis sp. nov., isolated from a soil sample. Antonie van Leeuwenhoek 110, 1149–1155 (2017). https://doi.org/10.1007/s10482-017-0887-6

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Keywords

  • Nocardia tengchongensis sp. nov.
  • 16S rRNA gene
  • Polyphasic taxonomy