Altererythrobacter lauratis sp. nov. and Altererythrobacter palmitatis sp. nov., isolated from a Tibetan hot spring

Abstract

Two Gram-negative, moderately thermophilic, yellow-pigmented, rod-shaped and motile bacterial strains, designated YIM 75003T and YIM 75004T, were isolated from sediment samples collected from the Tagejia hot spring in Tibet, western China. The taxonomic affiliation of the two strains was investigated by a polyphasic approach. Pairwise comparison of the 16S rRNA gene sequences showed that strains YIM 75003T and YIM 75004T are closely related to Altererythrobacter buctense M0322T (97.2 and 97.1% respectively), while sharing 99.9% sequence similarity against each other. Optimum growth of the two strains was observed at 37–45 °C, pH 8.0 and in the presence of 1–6% NaCl (w/v). Their predominant respiratory quinone was found to be ubiquinone 10. The major fatty acids in both the strains were identified as summed feature 8 (C18:1 ω6c and/or C18:1 ω7c) and summed feature 4 (C17:1 anteiso B and/or iso I). Their major polar lipid profiles were found to be diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid. The DNA G+C contents of strains YIM 75003T and YIM 75004T were determined to be 61.3 and 60.1 mol%, respectively. The DNA-DNA hybridization values between strains YIM 75003T and YIM 75004T, and between the two strains and their closely related phylogenetic neighbour A. buctense M0322T (=CGMCC 1.12871T) were less than 70%. Based on the morphological and physiological properties, phylogenetic analyses, chemotaxonomic characteristics and DNA–DNA relatedness values, the two strains can be distinguished from each other and from their phylogenetically closely related strain. Strains YIM 75003T and YIM 75004T are, therefore, considered to represent two novel species of the genus Altererythrobacter, for which the names Altererythrobacter lauratis sp. nov. (type strain YIM 75003T = KCTC 52606T = CCTCC AB2016268T) and Altererythrobacter palmitatis sp. nov. (type strain YIM 75004T = KCTC 52607T = CCTCC AB2016270T) are proposed.

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References

  1. Atlas RM (1997) Handbook of microbiological media, 2nd edn. CRC Press, New York

    Google Scholar 

  2. Buck JD (1982) Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl Environ Microbiol 44:992–993

    CAS  PubMed  PubMed Central  Google Scholar 

  3. Christensen H, Angen O, Mutters R, Olsen JE, Bisgaard M (2000) DNA–DNA hybridization determined in microwells using covalent attachment of DNA. Int J Syst Bacteriol 50:1095–1102

    CAS  Article  Google Scholar 

  4. Collins MD, Jones D (1980) Lipids in the classification and identification of coryneform bacteria containing peptidoglycan based on 2,4-diaminobutyric acid. J Appl Bacteriol 48:459–470

    CAS  Article  Google Scholar 

  5. Collins MD, Pirouz T, Goodfellow M, Minnikin DE (1977) Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230

    CAS  Article  PubMed  Google Scholar 

  6. Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution well as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229

    Article  Google Scholar 

  7. Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376

    CAS  Article  PubMed  Google Scholar 

  8. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  PubMed  Google Scholar 

  9. Fitch WM (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Biol 20:406–416

    Article  Google Scholar 

  10. Gonzalez C, Gutierrez C, Ramirez C (1978) Halobacterium vallismortis sp. nov., an amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. Can J Microbiol 24:710–715

    CAS  Article  PubMed  Google Scholar 

  11. Huang QY, Dong CZ, Dong RM, Jiang HC, Wang S, Wang GH, Fang B, Ding XX, Niu L, Li X, Zhang CL, Dong HL (2011) Archaeal and bacterial diversity in hot springs on the Tibetan Plateau, China. Extremophiles 15:549–563

    Article  PubMed  Google Scholar 

  12. Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    CAS  Article  PubMed  Google Scholar 

  13. Kovacs N (1956) Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178:703–704

    CAS  Article  PubMed  Google Scholar 

  14. Kroppenstedt RM (1982) Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Lip Chromatogr 5:2359–2367

    CAS  Article  Google Scholar 

  15. Kumar NR, Nair S, Langer S, Busse HJ, Kämpfer P (2008) Altererythrobacter indicus sp. nov., isolated from wild rice (Porteresia coarctata Tateoka). Int J Syst Evol Microbiol 58:839–844

    Article  PubMed  Google Scholar 

  16. Kwon KK, Woo JH, Yang SH, Kang JH, Kang SG, Kim SJ, Sato T, Kato C et al (2007) Altererythrobacter epoxidivorans gen. nov. sp. nov. an epoxide hydrolase-active, mesophilic marine bacterium isolated from cold-seep sediment, and reclassification of Erythrobacter luteolus Yoon, 2005 as Altererythrobacter luteolus comb. nov. Int J Syst Evol Microbiol 57:2207–2211

    CAS  Article  PubMed  Google Scholar 

  17. Li WJ, Xu P, Schuman P, Zhang YQ, Pukall R, Xu LH, Stackebrandt E, Jiang CL (2007) Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57:1424–1428

    Article  PubMed  Google Scholar 

  18. Liang XY, Lin HN, Wang KL, Liao YJ, Lai QL, Xu Y, Wang CY (2016) Altererythrobacter salegens sp. nov., a slightly halophilic bacterium isolated from surface sediment. Int J Syst Evol Microbiol. doi:10.1099/ijsem.0.001708

    Google Scholar 

  19. Locci R (1989) Streptomyces and related genera. In: Williams ST, Sharpe ME, Holt JG (eds) Bergey’s manual of systematic bacteriology, vol 4. Williams & Wilkins, Baltimore, pp 2451–2508

    Google Scholar 

  20. Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167

    CAS  Article  Google Scholar 

  21. Minnikin DE, Collins MD, Goodfellow M (1979) Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47:87–95

    CAS  Article  Google Scholar 

  22. Nedashkovskaya OI, Cho SH, Joung Y, Joh K, Kim MN, Shin KS, Oh HW, Bae KS, Mikhailov VV, Kim SB (2013) Altererythrobacter troitsensis sp. nov., isolated from the sea urchin Strongylocentrotus intermedius. Int J Syst Evol Microbiol 63:93–97

    CAS  Article  PubMed  Google Scholar 

  23. Park SC, Baik KS, Choe HN, Lim CH, Kim HJ, Ka JO, Seong CN (2011) Altererythrobacter namhicola sp. nov. and Altererythrobacter aestuarii sp. nov., isolated from seawater. Int J Syst Evol Microbiol 61:709–715

    CAS  Article  PubMed  Google Scholar 

  24. Rainey FA, Silva J, Nobre MF, Silva MT, Costa MS (2003) Porphyrobacter cryptus sp. nov., a novel slightly thermophilic, aerobic, bacteriochlorophyll a-containing species. Int J Syst Evol Microbiol 53:35–41

    CAS  Article  PubMed  Google Scholar 

  25. Reasoner DJ, Geldreich EE (1985) A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 49:1–7

    CAS  PubMed  PubMed Central  Google Scholar 

  26. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  27. Sasser M (2001) Identification of bacteria by gas chromatography of cellular fatty acids. http://www.microbialid.com/PDF/TechNote_101.pdf

  28. Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340

    Article  Google Scholar 

  29. Song ZQ, Zhi XY, Li WJ, Jiang HC, Zhang CL, Dong HL (2009) Actinobacterial diversity in hot springs in Tengchong (China), Kamachatka (Russia), Nevada (USA). J Gen Microbiol 26:256–263

    CAS  Google Scholar 

  30. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  31. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl Acids Res 25:4876–4882

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  32. Waksman SA (1967) The Actinomycetes: a summary of current knowledge. Ronald Press, New York

    Google Scholar 

  33. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE (1987) International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464

    Article  Google Scholar 

  34. Williams ST, Goodfellow M, Alderson G (1989) Genus Streptomyces Waksman and Henrici 1943, 339AL. In: Williams ST, Sharpe ME, Holt JG (eds) Bergey’s Manual of Systematic Bacteriology, vol. 4. Williams & Wilkins, Baltimore, pp 2453–2492

  35. Wu YH, Xu L, Meng FX, Zhang DS, Wang CS, Oren A, Xu XW (2014) Altererythrobacter atlanticus sp. nov., isolated from deep-sea sediment. Int J Syst Evol Microbiol 64:116–121

    CAS  Article  PubMed  Google Scholar 

  36. Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ, Chen HH, Xu H, Jiang CL (2005) Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family Oxalobacteraceae isolated from China. Int J Syst Evol Microbiol 55:1149–1153

    CAS  Article  PubMed  Google Scholar 

  37. Xue XQ, Zhang KD, Cai F, Dai J, Wang Y, Rahman E, Peng F, Fang CX (2012) Altererythrobacter xinjiangensis sp. nov., isolated from desert sand, and emended description of the genus Altererythrobacter. Int J Syst Evol Microbiol 62:28–32

    CAS  Article  PubMed  Google Scholar 

  38. Xue H, Piao CG, Guo MW, Wang LF, Fang W, Li Y (2016) Description of Altererythrobacter aerius sp. nov., isolated from air, and emended description of the genus Altererythrobacter. Int J Syst Evol Microbiol 66:4543–4548

    Article  PubMed  Google Scholar 

  39. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y, Seo H, Chun J (2016) Introducing EzBioCloud: a taxonomically united database of 16S rRNA and whole genome assemblies. Int J Syst Evol Microbiol. doi:10.1099/ijsem.0.001755

    Google Scholar 

  40. Zhang GY, Yang YL, Wang LN (2016a) Altererythrobacter aurantiacus sp. nov., isolated from deep-sea sediment. Antonie van Leeuwenhoek 109:1245–1251

    CAS  Article  PubMed  Google Scholar 

  41. Zhang W, Yuan X, Feng QQ, Zhang RG, Zhao XM (2016b) Lv J (2016b), Altererythrobacter buctense sp. nov., isolated from mudstone core. Antonie van Leeuwenhoek 109:793–799

    CAS  Article  PubMed  Google Scholar 

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Acknowledgements

We are grateful to Prof. Yu-Guang Zhou (CGMCC, China) for providing the reference type strain and Prof. Aharon Oren, the Hebrew University of Jerusalem, for suggesting the Latin names of the new strains. This work was supported by the Key Project of International Cooperation of Ministry of Science & Technology (MOST) (No. 2013DFA31980), Science and technology infrastructure work project (No. 2015FY110100) and project of China Tobacco Yunnan Industrial Co. Ltd. (No. 2015CP01). W.-J. Li was also supported by Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme (2014).

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Correspondence to Xiao-Yang Zhi or Wen-Jun Li.

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Yuan, CG., Chen, X., Jiang, Z. et al. Altererythrobacter lauratis sp. nov. and Altererythrobacter palmitatis sp. nov., isolated from a Tibetan hot spring. Antonie van Leeuwenhoek 110, 1077–1086 (2017). https://doi.org/10.1007/s10482-017-0882-y

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Keywords

  • Altererythrobacter lauratis sp. nov.
  • Altererythrobacter palmitatis sp. nov.
  • Polyphasic taxonomy
  • Tagejia hot spring