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Altererythrobacter lauratis sp. nov. and Altererythrobacter palmitatis sp. nov., isolated from a Tibetan hot spring

Antonie van Leeuwenhoek volume 110, pages 1077–1086 (2017)Cite this article

Abstract

Two Gram-negative, moderately thermophilic, yellow-pigmented, rod-shaped and motile bacterial strains, designated YIM 75003T and YIM 75004T, were isolated from sediment samples collected from the Tagejia hot spring in Tibet, western China. The taxonomic affiliation of the two strains was investigated by a polyphasic approach. Pairwise comparison of the 16S rRNA gene sequences showed that strains YIM 75003T and YIM 75004T are closely related to Altererythrobacter buctense M0322T (97.2 and 97.1% respectively), while sharing 99.9% sequence similarity against each other. Optimum growth of the two strains was observed at 37–45 °C, pH 8.0 and in the presence of 1–6% NaCl (w/v). Their predominant respiratory quinone was found to be ubiquinone 10. The major fatty acids in both the strains were identified as summed feature 8 (C18:1 ω6c and/or C18:1 ω7c) and summed feature 4 (C17:1 anteiso B and/or iso I). Their major polar lipid profiles were found to be diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid. The DNA G+C contents of strains YIM 75003T and YIM 75004T were determined to be 61.3 and 60.1 mol%, respectively. The DNA-DNA hybridization values between strains YIM 75003T and YIM 75004T, and between the two strains and their closely related phylogenetic neighbour A. buctense M0322T (=CGMCC 1.12871T) were less than 70%. Based on the morphological and physiological properties, phylogenetic analyses, chemotaxonomic characteristics and DNA–DNA relatedness values, the two strains can be distinguished from each other and from their phylogenetically closely related strain. Strains YIM 75003T and YIM 75004T are, therefore, considered to represent two novel species of the genus Altererythrobacter, for which the names Altererythrobacter lauratis sp. nov. (type strain YIM 75003T = KCTC 52606T = CCTCC AB2016268T) and Altererythrobacter palmitatis sp. nov. (type strain YIM 75004T = KCTC 52607T = CCTCC AB2016270T) are proposed.

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Acknowledgements

We are grateful to Prof. Yu-Guang Zhou (CGMCC, China) for providing the reference type strain and Prof. Aharon Oren, the Hebrew University of Jerusalem, for suggesting the Latin names of the new strains. This work was supported by the Key Project of International Cooperation of Ministry of Science & Technology (MOST) (No. 2013DFA31980), Science and technology infrastructure work project (No. 2015FY110100) and project of China Tobacco Yunnan Industrial Co. Ltd. (No. 2015CP01). W.-J. Li was also supported by Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme (2014).

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Author notes

  1. Chang-Guo Yuan, Xing Chen and Zhao Jiang have contributed equally to this work.

Affiliations

  1. Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Chang-Guo Yuan, Zhao Jiang, Xiao-Yang Zhi & Wen-Jun Li

  2. China Tobacco Yunnan Industrial Co. Ltd., Kunming, 650231, People’s Republic of China

    Xing Chen & Wei Chen

  3. State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

    Lan Liu, Wen-Dong Xian, Mipeshwaree Devi Asem & Wen-Jun Li

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  1. Chang-Guo Yuan
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  2. Xing Chen
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Correspondence to Xiao-Yang Zhi or Wen-Jun Li.

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Yuan, CG., Chen, X., Jiang, Z. et al. Altererythrobacter lauratis sp. nov. and Altererythrobacter palmitatis sp. nov., isolated from a Tibetan hot spring. Antonie van Leeuwenhoek 110, 1077–1086 (2017). https://doi.org/10.1007/s10482-017-0882-y

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Keywords

  • Altererythrobacter lauratis sp. nov.
  • Altererythrobacter palmitatis sp. nov.
  • Polyphasic taxonomy
  • Tagejia hot spring