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Screening lactic acid bacteria strains with ability to bind di-n-butyl phthalate via Turbiscan technique

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Abstract

Di-n-butyl phthalate (DBP) is a ubiquitous environmental contaminant that poses a risk to humans. Previous work indicates that the ability of lactic acid bacteria (LAB) to bind phthalic acid esters is strain-specific. As cell suspensions of LAB strains in aqueous solution are likely to be colloidal dispersions, this study provided a technique to efficiently screen LAB strains that bind DBP via Turbiscan, which has been widely used to measure the stability of emulsions or colloidal dispersions. Eleven LAB strains belonging to Lactobacillus plantarum, Lb. pentosus, Lb. paralimentarius, Lb. helveticus, Leuconostoc mesenteroides, Lb. acidophilus, Bifidobacterium lactis, and Bifidobacterium bifidum species were used in this study, and seven of them were selected to test in an earlier stage of exploring the process for finding a screening method; others were used for a validation test. It was observed that the various values of the 10 h Turbiscan Stability Index (TSI) of the cell suspension from each strain, at the equilibrium time of dispersed particles according to the peak thickness of cell-suspensions as measured by Turbiscan, had significant negative correlations with the DBP-binding percentage of LAB strains. Higher TSI values are correlated with lower binding of bacteria strains to DBP with a correlation coefficient of 0.8292. Cell surface hydrocarbons of LAB strains and their adherence were observed to correlate with DBP-binding percentages and may lead to the different states of aggregation or equilibrium of bacterial cell-suspensions, and the aggregation of bacterial cells resulted in fewer binding sites in the cell wall for DBP. Finally, four LAB strains were randomly selected to verify the feasibility of the method. In all, the findings demonstrate that TSI might be used as a tool to quickly screen strains that bind DBP. The present work could be extended to the removal of other toxic compounds, when screening of high-efficiency strains is required.

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Abbreviations

DBP:

Di-n-butyl phthalate

LAB:

Lactic acid bacteria

TSI:

Turbiscan Stability Index

CSH:

Cell surface hydrocarbons

PAEs:

Phthalate esters (PAEs)

AFB1:

Aflatoxin B1

HPLC:

High performance liquid chromatography

DMEM:

Dulbecco’s minimal essential medium

PBS:

Phosphate-buffered saline

MATH:

Microbial adhesion to hydrocarbons

LSD:

Least significant difference

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Acknowledgements

This work was supported by the Natural Science Foundation of China (No. 31471710) and the National High Technology Research and Development Program of The Ministry of Science and Technology of The People’s Republic of China (2011BAD23B04 and 2008AA10Z335). The authors cordially express thanks to Ms. He Yuwei, general manager of Beijing LDS Technology Co. Ltd., for permitting use of Turbiscan equipment and technical support.

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Authors and Affiliations

  1. College of Biological Science & Biotechnology, Beijing Forestry University, No. 35 Qinghua East Road Haidian District, Beijing, 100083, China

    Zhao Lili, Zhao Hongfei, Sana Shoukat, Zhang Xiaochen & Zhang Bolin

  2. Beijing Key Laboratory of Forest Food Processing and Safety, 100083, Beijing, China

    Zhao Hongfei & Zhang Bolin

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  1. Zhao Lili
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  2. Zhao Hongfei
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  3. Sana Shoukat
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  4. Zhang Xiaochen
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  5. Zhang Bolin
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Correspondence to Zhang Bolin.

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Lili, Z., Hongfei, Z., Shoukat, S. et al. Screening lactic acid bacteria strains with ability to bind di-n-butyl phthalate via Turbiscan technique. Antonie van Leeuwenhoek 110, 759–769 (2017). https://doi.org/10.1007/s10482-017-0846-2

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