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Identification and genomic analysis of antifungal property of a tomato root endophyte Pseudomonas sp. p21

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Abstract

Pseudomonas sp., which occupy a variety of ecological niches, have been widely studied for their versatile metabolic capacity to promote plant growth, suppress microbial pathogens, and induce systemic resistance in plants. In this study, a Pseudomonas sp. strain p21, which was isolated from tomato root endophytes, was identified as having antagonism against Aspergillus niger. Further analysis showed that this strain had the ability to biosynthesise siderophores and was less effective in inhibiting the growth of A. niger with the supplementation of Fe3+ in the agar medium. Genomic sequencing and the secondary metabolite cluster analysis demonstrated that Pseudomonas sp. p21 harboured 2 pyoverdine biosynthetic gene clusters, which encode compounds with predicted core structures and two variable tetra-peptide or eleven-peptide chains. The results indicated that siderophore-mediated competition for iron might be an important mechanism in Pseudomonas suppression of the fungal pathogen A. niger and in microbe-pathogen-plant interactions.

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Acknowledgements

This work was supported by Grants from the National Basic Research Program of China (973) (2013CB127504), the National Natural Science Foundation of China (Nos. 31170108; 31670125) and the Department of Science and Technology of Fujian Province (No. 2014J01111).

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    Authors and Affiliations

    1. Engineering Research Center of Industrial Microbiology of Ministry of Education, and College of Life Sciences, Fujian Normal University, Fuzhou, 350108, Fujian, China

      Rongqin Ma, Zhiqiang Cheng, Shaonan Lei, Xin Li & Baoyu Tian

    2. Key Laboratory of Molecular Genetics, Guizhou Academy of Tobacco Science, Guiyang, 550081, Guizhou, China

      Yi Cao

    3. Institute of Quality Standards and Testing Technology for Agro-Products, Fujian Academy of Agricultural Sciences, Fuzhou, 350003, Fujian, China

      Wei Huang & Yongkang Song

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    Rongqin Ma and Yi Cao contributed equally to this work.

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    Ma, R., Cao, Y., Cheng, Z. et al. Identification and genomic analysis of antifungal property of a tomato root endophyte Pseudomonas sp. p21. Antonie van Leeuwenhoek 110, 387–397 (2017). https://doi.org/10.1007/s10482-016-0811-5

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