Streptomyces xiangtanensis sp. nov., isolated from a manganese-contaminated soil

Abstract

An actinomycete strain, designated strain LUSFXJT, was isolated from a soil sample obtained near the Xiangtan Manganese Mine, Central-South China and characterised using a polyphasic taxonomic approach. The 16S rRNA gene sequence-based phylogenetic analysis indicated that this strain belongs to the genus Streptomyces. The DNA–DNA relatedness between this strain and two closely related type strains, Streptomyces echinatus CGMCC 4.1642T and Streptomyces lanatus CGMCC 4.137T, were 28.7 ± 0.4 and 19.9 ± 2.0%, respectively, values which are far lower than the 70% threshold for the delineation of a novel prokaryotic species. The DNA G+C content of strain LUSFXJT is 75.0 mol%. Chemotaxonomic analysis revealed that the menaquinones of strain LUSFXJT are MK-9(H6), MK-9(H8), MK-9(H2) and MK-8(H8). The polar lipid profile of strain LUSFXJT was found to contain diphosphatidylglycerol and an unidentified polar lipid. The major cellular fatty acids were identified as iso-C15:0, anteiso-C15:0, iso-C16:0, C16:0 and Summed feature 3. Strain LUSFXJT was found to contain meso-diaminopimelic acid as the diagnostic cell wall diamino acid and the whole cell hydrolysates were found to be rich in ribose, mannose and glucose. Based on phenotypic, phylogenetic and chemotaxonomic characteristics, it is concluded that strain LUSFXJT represents a novel species of the genus Streptomyces, for which the name S. xiangtanensis sp. nov. is proposed. The type strain is LUSFXJT (=GDMCC 4.133T = KCTC 39829T).

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Acknowledgements

The authors thank Ms. Song-Zhen Yang and Ms. Yong-Hong Wang (Guangdong Institute of Microbiology, Guangzhou, China) for providing excellent technical assistance. This work was supported by Scientific Research Project of Hunan Province Department of Education (16K032) and Chinese National Natural Sciences Foundation (31260556).

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Correspondence to Jian Gao.

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Mo, P., Yu, YZ., Zhao, JR. et al. Streptomyces xiangtanensis sp. nov., isolated from a manganese-contaminated soil. Antonie van Leeuwenhoek 110, 297–304 (2017). https://doi.org/10.1007/s10482-016-0797-z

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Keywords

  • Streptomyces xiangtanensis sp. nov.
  • Phylogeny
  • Polyphasic taxonomy