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Tessaracoccus defluvii sp. nov., isolated from an aeration tank of a sewage treatment plant

Abstract

A Gram-positive, non-motile, aerobic, coccus-shaped bacterium, designated strain LNB-140T, was isolated from a sewage treatment plant in the Republic of Korea and was characterised using a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence analysis showed that strain LNB-140T belongs to genus Tessaracoccus in the family Propionibacteriaceae of the phylum Actinobacteria. The 16S rRNA gene sequence similarities between strain LNB-140T and type strains of the genus, Tessaracoccus flavescens SST-39T and Tessaracoccus rhinocerotis YIM101269T are 97.8 and 97.4 %, respectively. The chemotaxonomic properties of strain LNB-140T are consistent with those of members of the genus Tessaracoccus: a quinone system with MK-9(H4) as the predominant menaquinone; anteiso-C15:0 and iso C15:0 as the predominant cellular fatty acids; and ll-2,6-diaminopimelic acid as the diagnostic peptidoglycan diamino acid. The major polar lipids were identified as diphosphatidylglycerol and phosphatidylethanolamine. The G+C content of the genomic DNA was determined to be 67.1 mol%. Differential phenotypic properties along with low DNA–DNA relatedness (<30 ± 3.2 %) with closely related type strains show that strain LNB-140T is distinct from previously described members of the genus Tessaracoccus and represents a novel species in this genus, for which the name Tessaracoccus defluvii sp. nov. is proposed. The type strain is LNB-140T (=KEMB 5401-076T = JCM 17540T).

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References

  • Cai M, Wang L, Cai H, Li Y, Wang YN, Tang YQ, Wu XL (2011) Salinarimonas ramus sp. nov., and Tessaracoccus oleiagri sp. nov., isolated from a crude oil-contaminated saline soil. Int J Syst Evol Microbiol 61:1767–1775

    CAS  Article  PubMed  Google Scholar 

  • Cappuccino JG, Sherman N (2002) Microbiology: a laboratory manual, 6th edn. Benjamin Cummings, San Francisco

    Google Scholar 

  • Collins MD, Jones D (1981) Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implications. Microbiol Rev 45:316–354

    CAS  PubMed  PubMed Central  Google Scholar 

  • Doetsch RN (1981) Determinative methods of light microscopy. In: Gerhardt P, Murray RGE, Costilow RN, Nester EW, Wood WA, Krieg NR, Phillips GH (eds) Manual of methods for general bacteriology. American Society for Microbiology, Washington, DC, pp 21–33

    Google Scholar 

  • Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric DNA–DNA hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229

    Article  Google Scholar 

  • Felsenstein J (1985) Confidence limit on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Finster KW, Cockell CS, Voytek MA, Gronstal AL, Kjeldsen KU (2009) Description of Tessaracoccus profundi sp. nov., a deep-subsurface actinobacterium isolated from a Chesapeake impact crater drill core (940 m depth). Antonie Van Leeuwenhoek 96:515–526

    CAS  Article  PubMed  Google Scholar 

  • Fitch WM (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416

    Article  Google Scholar 

  • Frank JA, Reich CI, Sharma S et al (2008) Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genes. Appl Environ Microbiol 74:2461–2470

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  • Kämpfer P, Lodders N, Warfolomeow I, Busse HJ (2009) Tessaracoccus lubricantis sp. nov., isolated from a metalworking fluid. Int J Syst Evol Microbiol 59:1545–1549

    Article  PubMed  Google Scholar 

  • Kimura M (1983) The Neutral theory of molecular evolution. Cambridge University Press, Cambridge

    Book  Google Scholar 

  • Kumari R, Singh P, Schumann P, Lal R (2016) Tessaracoccus flavus sp. nov., isolated from the drainage system of a lindane producing factory. Int J Syst Bacteriol. doi:10.1099/ijsem.0.000958

    Google Scholar 

  • Lee DW, Lee SD (2008) Tessaracoccus flavescens sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 58:785–789

    Article  PubMed  Google Scholar 

  • Li GD, Chen X, Li QY, Xu FJ et al (2016) Tessaracoccus rhinocerotis sp. nov., isolated from the faeces of Rhinoceros unicornis. Int J Syst Bacteriol 66:922–927

    CAS  Article  Google Scholar 

  • Maszenan AM, Seviour RJ, Patel BKC, Schumann P, Rees GN (1999) Tessaracoccus bendigoensis gen. nov., sp. nov., a Gram-positive coccus occurring in regular packages or tetrads, isolated from activated sludge biomass. Int J Syst Bacteriol 49:459–468

    CAS  Article  PubMed  Google Scholar 

  • Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167

    CAS  Article  Google Scholar 

  • Minnikin DE, Patel PV, Alshamaony L, Goodfellow M (1977) Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117

    CAS  Article  Google Scholar 

  • Saitou N, Nei M (1987) The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Bio Evol 4:406–425

    CAS  Google Scholar 

  • Sánchez FP, Román MS, Amils R, Parro V (2014) Tessaracoccus lapidicaptus sp. nov., an actinobacterium isolated from the deep subsurface of the Iberian pyrite belt. Int J Syst Bacteriol 64:3546–3552

    Article  Google Scholar 

  • Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI technical note 101. MIDI Inc, Newark, DE

  • Schleifer KH, Kandler O (1972) Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477

    CAS  PubMed  PubMed Central  Google Scholar 

  • Seck E, Traore SI, Khelaifia S, Beye M, Michelle C, Couderc C, Brah S, Fournier P-E, Raoult D, Dubourg G (2016) Tessaracoccus massiliensis sp. nov., a new bacterial species isolated from the human gut. New Microbes New Infect 13:3–12

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  • Tamaoka J, Komagata K (1984) Determination of DNA base composition by reversed phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128

    CAS  Article  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28(10):2731–2739

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  • Teti D, Visalli M, Mcnair H (2002) Analysis of polyamines as markers of (patho) physiological conditions. J Chromatogr B 781:107–149

    CAS  Article  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882

    Article  Google Scholar 

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Acknowledgments

This research was supported by a Grant (14CTAP-C078666-01) from Infrastructure and transportation technology promotion research Program funded by Ministry of Land, infrastructure and Transport of Korean government and Korea Environmental Microorganisms Bank (NRF-2015M3A9B8029697).

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Correspondence to Sang-Seob Lee.

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Sathiyaraj Srinivasan and Aravind Sundararaman have contributed equally.

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Srinivasan, S., Sundararaman, A. & Lee, SS. Tessaracoccus defluvii sp. nov., isolated from an aeration tank of a sewage treatment plant. Antonie van Leeuwenhoek 110, 1–9 (2017). https://doi.org/10.1007/s10482-016-0766-6

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  • DOI: https://doi.org/10.1007/s10482-016-0766-6

Keywords

  • Taxonomy
  • Tessaracoccus
  • Sewage treatment plant