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Tessaracoccus defluvii sp. nov., isolated from an aeration tank of a sewage treatment plant

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Abstract

A Gram-positive, non-motile, aerobic, coccus-shaped bacterium, designated strain LNB-140T, was isolated from a sewage treatment plant in the Republic of Korea and was characterised using a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence analysis showed that strain LNB-140T belongs to genus Tessaracoccus in the family Propionibacteriaceae of the phylum Actinobacteria. The 16S rRNA gene sequence similarities between strain LNB-140T and type strains of the genus, Tessaracoccus flavescens SST-39T and Tessaracoccus rhinocerotis YIM101269T are 97.8 and 97.4 %, respectively. The chemotaxonomic properties of strain LNB-140T are consistent with those of members of the genus Tessaracoccus: a quinone system with MK-9(H4) as the predominant menaquinone; anteiso-C15:0 and iso C15:0 as the predominant cellular fatty acids; and ll-2,6-diaminopimelic acid as the diagnostic peptidoglycan diamino acid. The major polar lipids were identified as diphosphatidylglycerol and phosphatidylethanolamine. The G+C content of the genomic DNA was determined to be 67.1 mol%. Differential phenotypic properties along with low DNA–DNA relatedness (<30 ± 3.2 %) with closely related type strains show that strain LNB-140T is distinct from previously described members of the genus Tessaracoccus and represents a novel species in this genus, for which the name Tessaracoccus defluvii sp. nov. is proposed. The type strain is LNB-140T (=KEMB 5401-076T = JCM 17540T).

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Acknowledgments

This research was supported by a Grant (14CTAP-C078666-01) from Infrastructure and transportation technology promotion research Program funded by Ministry of Land, infrastructure and Transport of Korean government and Korea Environmental Microorganisms Bank (NRF-2015M3A9B8029697).

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    Authors and Affiliations

    1. Department of Life Science, Kyonggi University, Suwon-si, Gyeonggi-do, 442-760, Republic of Korea

      Sathiyaraj Srinivasan, Aravind Sundararaman & Sang-Seob Lee

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    1. Sathiyaraj Srinivasan
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    2. Aravind Sundararaman
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    3. Sang-Seob Lee
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    Correspondence to Sang-Seob Lee.

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    Sathiyaraj Srinivasan and Aravind Sundararaman have contributed equally.

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    Srinivasan, S., Sundararaman, A. & Lee, SS. Tessaracoccus defluvii sp. nov., isolated from an aeration tank of a sewage treatment plant. Antonie van Leeuwenhoek 110, 1–9 (2017). https://doi.org/10.1007/s10482-016-0766-6

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