A Gram-stain positive, facultatively anaerobic, non-motile and rod to coccoid-shaped bacterium, designated XZ17T, was isolated from Namtso Lake of Tibet, China. Strain XZ17T grew optimally at pH 8.0–9.0, at 30–33 °C and in the presence of 0–1.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that strain XZ17T belongs to the genus Nocardioides in the family Nocardioidaceae. Strain XZ17T shared pairwise 16S rRNA gene sequence similarities of 97.2, 96.8, 96.5, 96.4 and <96.0 % to Nocardioides solisilvae KCTC 39528T, Nocardioides daejeonensis JCM 16922T, Nocardioides jensenii NCIB 9770T, Nocardioides dubius KCTC 9992T and other Nocardioides species, respectively. It contained MK-8 (H4) as the predominant menaquinone and C17:1 ω8c, C15:0, C17:0 and C18:1 ω9c as the major fatty acid. The strain had cell wall peptidoglycan based on ll-2,6-diaminopimelic acid. The polar lipids of strain XZ17T comprised diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, three unidentified glycolipids and two unidentified lipids. The DNA G+C content of strain XZ17T was 68.9 mol%. Based on distinctive phenotypic characteristics, phylogenetic analysis and chemotaxonomic data, it can be concluded that strain XZ17T represents a novel species within the genus Nocardioides, for which the name Nocardioides gilvus sp. nov. is proposed. The type strain is strain XZ17T (=KCTC 39561T = MCCC 1H00114T).
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This work was supported by the National Natural Science Foundation of China (31370057, 31290231) and 2013 Shandong Provincial Second Group Projects on Resource Platforms for Marine Economic and Innovative Development Regions: Marine Microorganisms Preservation Platform (2150299).
The GenBank accession number for the 16S rRNA gene sequence of strain XZ17T is KR534267.
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Zhang, HX., Wang, K., Xu, ZX. et al. Nocardioides gilvus sp. nov., isolated from Namtso Lake. Antonie van Leeuwenhoek 109, 1367–1374 (2016). https://doi.org/10.1007/s10482-016-0735-0
- Nocardioides gilvus sp. nov.
- 16S rRNA gene
- Namtso Lake