Antonie van Leeuwenhoek

, Volume 109, Issue 10, pp 1307–1322 | Cite as

Actinobacteria possessing antimicrobial and antioxidant activities isolated from the pollen of scots pine (Pinus sylvestris) grown on the Baikal shore

  • Denis V. Axenov-Gribanov
  • Irina V. Voytsekhovskaya
  • Yuriy V. Rebets
  • Bogdan T. Tokovenko
  • Tatyana A. Penzina
  • Tatyana G. Gornostay
  • Renat V. Adelshin
  • Eugenii S. Protasov
  • Andriy N. Luzhetskyy
  • Maxim A. Timofeyev
Original Article


Isolated ecosystems existing under specific environmental conditions have been shown to be promising sources of new strains of actinobacteria. The taiga forest of Baikal Siberia has not been well studied, and its actinobacterial population remains uncharacterized. The proximity between the huge water mass of Lake Baikal and high mountain ranges influences the structure and diversity of the plant world in Siberia. Here, we report the isolation of eighteen actinobacterial strains from male cones of Scots pine trees (Pinus sylvestris) growing on the shore of the ancient Lake Baikal in Siberia. In addition to more common representative strains of Streptomyces, several species belonging to the genera Rhodococcus, Amycolatopsis, and Micromonospora were isolated. All isolated strains exhibited antibacterial and antifungal activities. We identified several strains that inhibited the growth of the pathogen Candida albicans but did not hinder the growth of Saccharomyces cerevisiae. Several isolates were active against Gram-positive and Gram-negative bacteria. The high proportion of biologically active strains producing antibacterial and specific antifungal compounds may reflect their role in protecting pollen against phytopathogens.


Actinobacteria Baikal Lake Biodiversity Secondary metabolites Pinus sylvestris Amycolatopsis sp. Rhodococcus sp. Male cones Pollen 


Author contributions

Conceived and designed the experiments: DAG, IV, YR, MT; Performed the experiments: DAG IV; Analyzed the data: DAG, BT, TG, IV, RA; Contributed reagents/materials/analysis tools: MT, AL, TP; Wrote the manuscript: DAG, YR, BT, MT, AL; Other: Sample collection: EP.


This paper is dedicated to Irkutsk regional veterinary laboratory where the inhibiting influence of crude extracts were estimated against C. albicals.

This study was supported by the Ministry of education and science of Russian Federation as a part of Goszadanie projects (№6.382.2014/K, 6.734.2016 DAAD, 6.696.2016 DAAD), Russian science foundation (project N 14-14-00400), Russian foundation for basic research (projects N 14-04-00501, 15-54-04062), Grants of Irkutsk State University for researchers and Deutscher Akademischer Austauschdienst.

Compliance with ethical standards


This study was supported by the Ministry of education and science of Russian Federation as a part of Goszadanie projects (№6.382.2014/K, 6.734.2016 DAAD, 6.696.2016 DAAD), Russian science foundation (project N 14-14-00400), Russian foundation for basic research (projects N 14-04-00501, 15-54-04062), Grants of Irkutsk State University for researchers and Deutscher Akademischer Austauschdienst.

Conflict of Interest

The authors declare that they have no conflict of interest.

Statement of human rights

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Statement on the welfare of animals

This article does not contain any studies with human participants or vertebrate animals performed by any of the authors.

Mentioned in the article plant species not involve in endangered or protected species. No specific permissions were required for sampling.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Supplementary material

10482_2016_730_MOESM1_ESM.docx (384 kb)
Supplementary material 1 (DOCX 383 kb)


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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Denis V. Axenov-Gribanov
    • 1
  • Irina V. Voytsekhovskaya
    • 1
  • Yuriy V. Rebets
    • 2
  • Bogdan T. Tokovenko
    • 2
  • Tatyana A. Penzina
    • 1
    • 4
  • Tatyana G. Gornostay
    • 4
  • Renat V. Adelshin
    • 1
    • 5
  • Eugenii S. Protasov
    • 1
  • Andriy N. Luzhetskyy
    • 2
    • 3
  • Maxim A. Timofeyev
    • 1
  1. 1.Institute of Biology at Irkutsk State UniversityIrkutskRussia
  2. 2.Helmholtz Institute for Pharmaceutical Research SaarlandSaarbruckenGermany
  3. 3.Pharmazeutische BiotechnologieUniversität des SaarlandesSaarbruckenGermany
  4. 4.Siberian Institute of Plant Physiology and Biochemistry SB RASIrkutskRussia
  5. 5.Irkutsk Anti-Plague Research Institute of Siberia and Far EastIrkutskRussia

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