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Rare or rarely detected? Ceraceosorus guamensis sp. nov.: a second described species of Ceraceosorales and the potential for underdetection of rare lineages with common sampling techniques

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Abstract

Ceraceosorales is a monotypic order in Ustilaginomycotina. Its namesake, Ceraceosorus bombacis, was described as a phytopathogen of Bombax ceiba in India. In this study, we describe Ceraceosorus guamensis sp. nov., collected on the South Pacific island of Guam, which appears to represent the second isolation of any member of this order in over 40 years. Ceraceosorus species are monokaryotic and filamentous in culture, producing conidia on potato dextrose agar. However, both species behave yeast-like when cultured on corn meal agar. The internal transcribed spacer (ITS) region (spanning the ITS1–5.8S–ITS2) in both species of Ceraceosorus is highly heterogeneous containing multiple disparate copies that can vary intragenomically by up to 3.5 %. Moreover, this region could not be amplified using the fungal ITS primers most frequently used for culture-independent methods of assessing fungal biodiversity. This fact, combined with the extremely slow growth rates on commonly employed media, may indicate that members of this lineage are potentially underdetected by current sampling methods.

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Acknowledgments

We thank the Plant and Pest Diagnostic Laboratory (PPDL) for microplate facilities, and the lab of Dr. Jin-Rong Xu for microscope facilities. Dr. Robert Schlub is kindly acknowledged for facilitating collecting on Guam by MCA. TK study at Purdue University is funded by an Anadamahidol Foundation Scholarship, Thailand. Finally, we would like to thank John F. Klimek and all members of the Aime lab for help in culture maintenance and molecular lab support, and three anonymous reviewers for helpful comments on an earlier version of this manuscript.

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  1. Department of Botany and Plant Pathology, Purdue University, 915 W State St, West Lafayette, IN, 47907-2054, USA

    Teeratas Kijpornyongpan & M. Catherine Aime

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  1. Teeratas Kijpornyongpan
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Correspondence to M. Catherine Aime.

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Kijpornyongpan, T., Catherine Aime, M. Rare or rarely detected? Ceraceosorus guamensis sp. nov.: a second described species of Ceraceosorales and the potential for underdetection of rare lineages with common sampling techniques. Antonie van Leeuwenhoek 109, 1127–1139 (2016). https://doi.org/10.1007/s10482-016-0715-4

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