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Antonie van Leeuwenhoek

, Volume 109, Issue 8, pp 1101–1109 | Cite as

Nocardioides pakistanensis sp. nov., isolated from a hot water spring of Tatta Pani in Pakistan

  • Arshia Amin
  • Iftikhar Ahmed
  • Neeli Habib
  • Saira Abbas
  • Min Xiao
  • Wael N. Hozzein
  • Wen-Jun Li
Original Paper

Abstract

A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium, designated as NCCP-1340T, was isolated from a hot water spring, Tatta Pani, Pakistan. Cells of strain NCCP-1340T were observed to be aerobic, rod shaped, catalase and urease positive but H2S production and oxidase negative. Growth was observed at pH 6.0–8.0 (optimum pH 7.0) and at 20–40 °C (optimum 37 °C). The strain could tolerate 0–8 % NaCl (optimum 2 %, w/v). Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain NCCP-1340T belongs to the genus Nocardioides and is closely related to Nocardioides iriomotensis JCM 17985T (96.8 %), Nocardioides daedukensis KCTC 19601T (96.6 %), Nocardioides jensenii KCTC 9134T (96.1 %) and Nocardioides daejeonensis KCTC 19772T (96.1 %). The DNA–DNA relatedness values of strain NCCP-1340T with N. iriomotensis JCM 17985T, N. daedukensis KCTC 19601T and N. jensenii KCTC 9134T were found to be less than 53 %. The DNA G+C content of strain NCCP-1340T was determined to be 71.8 mol  %. The affiliation of strain NCCP-1340T to the genus Nocardioides was further supported by chemotaxonomic data which showed the presence of MK-8(H4) as major menaquinone system; iso-C16:0, C17:0, C16:0 10-methyl, iso-C15:0 and C 15:0 as major cellular fatty acids; and diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and unidentified glycolipids and polar lipids in the polar lipids profile. The cell wall peptidoglycan contained LL-diaminopimelic acid as the diagnostic amino acid. On the basis of physiological and biochemical characteristics and the phylogenetic analyses, strain NCCP-1340T can be distinguished from the closely related taxa and thus represents a novel species of the genus Nocardioides, for which the name Nocardioides pakistanensis sp. nov. is proposed with the type strain NCCP-1340T (= DSM 29942T = JCM 30630T).

Keywords

Nocardioidespakistanensis sp. nov. Hot water spring Tatta Pani Pakistan 

Notes

Acknowledgments

We are grateful to Prof. Takuji Kudo (JCM, Japan) and Miss Sang-Mi Lee (KCTC, Korea) for kindly providing the reference type strains. This work was supported by the Key Project of International Cooperation of Ministry of Science & Technology (MOST) (No. 2013DFA31980), Science and technology infrastructure work project (No. 2015FY110100) and the Deanship of Scientific Research at King Saud University (through the research group no. 1436-27). W-J Li was also supported by Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme (2014).

Supplementary material

10482_2016_711_MOESM1_ESM.doc (450 kb)
Supplementary material 1 (DOC 451 kb)

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Arshia Amin
    • 1
    • 2
    • 3
  • Iftikhar Ahmed
    • 2
    • 4
  • Neeli Habib
    • 1
  • Saira Abbas
    • 2
    • 4
  • Min Xiao
    • 5
  • Wael N. Hozzein
    • 6
  • Wen-Jun Li
    • 1
    • 5
  1. 1.Yunnan Institute of MicrobiologyYunnan UniversityKunmingPeople’s Republic of China
  2. 2.Institute of Microbial Culture Collection of Pakistan (IMCCP)National Agricultural Research Centre (NARC)IslamabadPakistan
  3. 3.Department of MicrobiologyQuaid-i-Azam UniversityIslamabadPakistan
  4. 4.PARC Institute of Advance Studies in Agriculture (PIASA)National Agricultural Research Centre (NARC)IslamabadPakistan
  5. 5.State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life SciencesSun Yat-Sen UniversityGuangzhouPeople’s Republic of China
  6. 6.Bioproducts Research Chair (BRC) College of ScienceKing Saud UniversityRiyadhSaudi Arabia

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