Thalassospira australica sp. nov. isolated from sea water
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Two Gram-negative, non-pigmented, motile bacteria were isolated from a sea water sample collected at St. Kilda Beach, Port Philip Bay, Victoria, Australia. The two strains were found to grow between 4 and 40 °C, pH 5–10 and tolerate up to 10 % NaCl. A phylogenetic study, based on a 16S rRNA gene sequence analysis indicated that strains NP 3b2T and H 94 belong to the genus Thalassospira. The sequence similarity of the 16S rRNA gene between the two new isolates is 99.8 % and between these strains and all validly named Thalassospira species was found to be in the range of 95–99.4 %. The DNA–DNA relatedness between the two strains was found to be 80.2 %, while relatedness with other validly named species of the genus Thalassospira was between 53 and 65 %. The average nucleotide identity (ANI) and the in silico genome-to-genome distance (GGD) between the two bacteria and T. profundimaris WP0211T, T. xiamenensis M-5T, ‘T. permensis’ NBRC 106175T and T. lucentensis QMT2T was 76–82 % and 21–25 %, respectively. The results of phylogenetic and genomic analysis, together with physiological and biochemical properties, indicated that the two strains represent a new species of the genus Thalassospira. Based on these data, a new species, Thalassospira australica, is proposed with strain NP 3b2T (=KMM 6365T = JCM 31222T) as the type strain.
KeywordsThalassospira Marine bacteria Taxonomy Phylogeny Genomic taxonomy New species
Financial support was provided by the Russian Science Foundation (Contract No. 14-50-00034) to N.V. Zhukova for lipid analysis.
Conflict of interest
The authors declare that they have no conflict of interest.
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