Oceanobacillus endoradicis sp. nov., an endophytic bacterial species isolated from the root of Paris polyphylla Smith var. yunnanensis

Abstract

A bacterial strain, py1294T, isolated from a root of Paris polyphylla Smith var. yunnanensis collected from Yunnan province, southwest China, was characterised by using a polyphasic approach to clarify its taxonomic position. Strain py1294T was found to be Gram-positive, aerobic, spore-forming, peritrichous flagella and rod shaped. Growth was found to occur in the presence of 0–8 % (w/v) NaCl (optimum 1–3 %), at pH 6.5–9.5 (optimum 8.0) and at 10–42 °C (optimum 30 °C). The major cellular fatty acids were identified as anteiso-C15:0, anteiso-C17:0, iso-C16:0 and iso-C14:0. The predominant quinone was identified as MK-7 and a minor amount of MK-6 was detected. The diagnostic polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The cell wall peptidoglycan was found to contain meso-diaminopimelic acid. Phylogenetic analysis of the 16S rRNA gene sequence showed that strain py1294T forms a well-supported clade with Oceanobacillus damuensis PT-20T (97.9 % sequence similarity) within the genus Oceanobacillus, although it also shares a high sequence similarity with Ornithinibacillus contaminans (97.5 %). Crucially, the DNA–DNA relatedness value between strain py1294T and O. damuensis PT-20T was 29.7 ± 3.2 %. The G+C content was determined to be 42.3 mol%. On the basis of the phylogenetic and phenotypic data, a novel species Oceanobacillus endoradicis sp. nov. is proposed, with py1294T (=DSM 100726T = KCTC 33731T) as the type strain.

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (Grant Numbers: 31500007 and 31560309), and the opening project of the State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences (Grant Number: SKLMR-20140601). We are grateful to Dr. Yongqiang Tian of Sichuan University for providing the type strain of O. damuensis PT-20T.

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Correspondence to Xiao-Yang Zhi.

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Yang, LL., Tang, SK., Chu, X. et al. Oceanobacillus endoradicis sp. nov., an endophytic bacterial species isolated from the root of Paris polyphylla Smith var. yunnanensis . Antonie van Leeuwenhoek 109, 957–964 (2016). https://doi.org/10.1007/s10482-016-0695-4

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Keywords

  • Oceanobacillus endoradicis sp. nov.
  • Polyphasic taxonomy
  • Paris polyphylla Smith var. yunnanensis
  • Endophytes