Description of Kibdelosporangium banguiense sp. nov., a novel actinomycete isolated from soil of the forest of Pama, on the plateau of Bangui, Central African Republic

Abstract

A novel actinomycete strain F-240,109T from the MEDINA collection was isolated from a soil sample collected in the forest of Pama, on the plateau of Bangui, Central African Republic. The strain was identified according to its 16S rRNA gene sequence as a new member of the genus Kibdelosporangium, being closely related to Kibdelosporangium aridum subsp. aridum (98.6 % sequence similarity), Kibledosporangium phytohabitans (98.3 %), Kibdelosporangium aridum subsp. largum (97.7 %), Kibdelosporangium philippinense (97.6 %) and Kibledosporangium lantanae (96.9 %). In order to resolve its precise taxonomic status, the strain was characterised through a polyphasic approach. The strain is a Gram-stain positive, aerobic, non-motile and catalase-positive actinomycete characterised by formation of extensively branched substrate mycelia and sparse brownish grey aerial mycelia with sporangium-like globular structures. The chemotaxonomic characterisation of strain F-240,109T corroborated its affiliation into the genus Kibdelosporangium. The peptidoglycan contains meso-diaminopimelic acid; the major menaquinone is MK-9(H4); the phospholipid profile contains high amounts of phosphatidylethanolamine, hydroxyphosphatidylethanolamine, diphosphatidylglycerol and an unidentified phospholipid; and the predominant cellular fatty acid methyl esters are iso-C16:0, iso-C14:0, iso-C15:0 and 2OH iso-C16:0. However, some key phenotypic differences regarding to its close relatives and DNA–DNA hybridization values indicate that strain F-240,109T represents a novel Kibdelosporangium species, for which the name Kibdelosporangium banguiense sp. nov. is proposed. The type strain is strain F-240,109T (=DSM 46670T, =LMG 28181T).

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Acknowledgments

This research was supported by the Non-Oriented Basic Research Program of the Spanish Ministry of Economy (Project SAF2010-15010). The authors thank Professor Dr. Bernhard Schink for his advice on the nomenclature of Kibdelosporangium banguiense sp. nov.

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Correspondence to Olga Genilloud.

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Pascual, J., González, I., Estévez, M. et al. Description of Kibdelosporangium banguiense sp. nov., a novel actinomycete isolated from soil of the forest of Pama, on the plateau of Bangui, Central African Republic. Antonie van Leeuwenhoek 109, 685–695 (2016). https://doi.org/10.1007/s10482-016-0669-6

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Keywords

  • Kibdelosporangium banguiense sp. nov.
  • Polyphasic taxonomy
  • 16S rRNA
  • Actinomycetes
  • Pseudonocardiaceae