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Antonie van Leeuwenhoek

, Volume 109, Issue 5, pp 611–618 | Cite as

Roseomonas chloroacetimidivorans sp. nov., a chloroacetamide herbicide-degrading bacterium isolated from activated sludge

  • Cui-Wei Chu
  • Qing Chen
  • Cheng-Hong Wang
  • Hong-Mei Wang
  • Zhong-Guan Sun
  • Qin He
  • Jian He
  • Jin-Gang Gu
Original Paper

Abstract

A Gram-negative, aerobic, short rod-shaped, pink-pigmented, non-motile bacterium, designated BUT-13T, was isolated from activated sludge of an herbicide-manufacturing wastewater treatment facility in Jiangsu province, China. Growth was observed at 0–5.5 % NaCl, pH 6.0–9.0 and 12–37 °C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain BUT-13T is a member of the genus Roseomonas, and shows high sequence similarities to R. pecuniae N75T (98.0 %) and R. rosea 173-96T (97.5 %), and lower (<97 %) sequence similarities to all other Roseomonas species. Chemotaxonomic analysis revealed that strain BUT-13T possesses Q-10 as the predominant ubiquinone; summed feature 8 (C18:1 w7c and/or C18:1 w6c; 38.8 %), C18:0 (16.6 %), C16:0 (15.2 %), summed feature 3 (C16:1 ω6c and/or C16:1 ω7; 7.9 %) and C18:1 w9c (4.7 %) as the major fatty acids. The polar lipids were found to consist of two aminolipids, a glycolipid, a phospholipid, a phosphoglycolipid, phosphatidylcholine, phosphatidylethanolamine and diphosphatidylglycerol. Strain BUT-13T showed low DNA–DNA relatedness with R. pecuniae N75T (45.2 %) and R. rosea 173-96T (51.2 %). The DNA G+C content was determined to be 67.6 mol%. Based on the phylogenetic analysis, DNA–DNA hybridization and chemotaxonomic analysis, as well as biochemical characteristics, strain BUT-13T can be clearly distinguished from all currently recognised Roseomonas species and should be classified as a novel species of the genus Roseomonas, for which the name Roseomonas chloroacetimidivorans sp. nov. is proposed. The type strain is BUT-13T (CCTCC AB 2015299T = JCM 31050T).

Keywords

Roseomonas chloroacetimidivorans sp. nov Polyphasic taxonomy Degrading 

Notes

Acknowledgments

This work was supported by the National science and technology support plan (2013AA102804), the National Natural Science Foundation of China (Grant no. 31270157, 31560033), the Program for New Century Excellent Talents in University (NCET-13-0861) and the Research Fund for the Doctoral Program of Zaozhuang University, China (2014BS14).

Supplementary material

10482_2016_664_MOESM1_ESM.docx (11 kb)
Supplementary material 1 (DOCX 10 kb)

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Cui-Wei Chu
    • 1
  • Qing Chen
    • 2
  • Cheng-Hong Wang
    • 3
  • Hong-Mei Wang
    • 2
  • Zhong-Guan Sun
    • 2
  • Qin He
    • 1
  • Jian He
    • 1
  • Jin-Gang Gu
    • 4
  1. 1.Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life SciencesNanjing Agricultural UniversityNanjingPeople’s Republic of China
  2. 2.College of Life SciencesZaozhuang UniversityZaozhuangPeople’s Republic of China
  3. 3.Key Laboratory of Systems Biology Medicine of Jiangxi Province, College of Basic Medical ScienceJiujiang UniversityJiujiangPeople’s Republic of China
  4. 4.Agricultural Culture Collection of China, Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijingPeople’s Republic of China

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