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Ascidiimonas aurantiaca gen. nov., sp. nov., a member of Flavobacteriaceae isolated from a sea squirt

Antonie van Leeuwenhoek volume 109, pages 501–508 (2016)Cite this article

Abstract

A Gram-stain negative, strictly aerobic, chemoheterotrophic, tangerine orange pigmented, curved-rod shaped bacterium, designated N5DA8-2CT, was isolated from a sea squirt by use of a bait-streaked agar technique. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the novel marine strain is affiliated with the family Flavobacteriaceae of the phylum Bacteroidetes and that it shared high (92.6 %) sequence similarity with Frondibacter aureus A5Q-67T. The strain could be differentiated phenotypically from the related members of the family Flavobacteriaceae. The major fatty acids of strain N5DA8-2CT were iso-C15:1 G, iso-C17:0 3-OH, iso-C15:0 and C16:1 ω7c and/or C16:1 ω6c. A polar lipid profile was present consisting of phosphatidylethanolamine and three unidentified amino lipids. The DNA G+C content of the strain was determined to be 41 mol% and the major respiratory quinone was identified as menaquinone 6 (MK-6). From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, the strain is considered to represent a novel genus in the family Flavobacteriaceae, for which the name Ascidiimonas aurantiaca gen. nov., sp. nov. is proposed. The type strain of A. aurantiaca gen. nov., sp. nov. is N5DA8-2CT (= KCTC 32992T = NBRC 110020T).

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Authors and Affiliations

  1. College of Pharmacy, Keimyung University, 1095 Dalgubeoldaero, Dalseo-Gu, Daegu, 42601, Republic of Korea

    Jaewoo Yoon

  2. Biotechnology Research Center, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama, 939-0398, Japan

    Naoya Oku

  3. Marine Biosciences Kamaishi Research Laboratory, Kitasato University, 160-4 Utou, Okirai, Sanriku-cho, Ofunato, Iwate, 022-0101, Japan

    Hiroaki Kasai

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  1. Jaewoo Yoon
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  2. Naoya Oku
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Correspondence to Jaewoo Yoon.

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10482_2016_655_MOESM1_ESM.ppt

Supplementary Fig. 1. Thin-layer chromatograms showing the total polar lipid compositions of N5DA8-2CT. Total polar lipids were detected by spraying the plate with molybdophosphoric acid, molybdenun blue, α-naphthol and ninhydrin. PE: phosphatidylenthanolamine, UAL: unidentified aminolipid

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Yoon, J., Oku, N. & Kasai, H. Ascidiimonas aurantiaca gen. nov., sp. nov., a member of Flavobacteriaceae isolated from a sea squirt. Antonie van Leeuwenhoek 109, 501–508 (2016). https://doi.org/10.1007/s10482-016-0655-z

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Keywords

  • Bacteroidetes
  • Flavobacteriaceae
  • Ascidiimonas aurantiaca gen. nov., sp. nov.
  • Sea anemone
  • Bait-streaked agar technique
  • 16S rRNA gene
  • Polyphasic taxonomy